Biotech Support Group Release: Lipid Removal From Docetaxel-Prodrug In Perfluorocarbon Nanoparticles By Sample Pretreatment Using Cleanascite
Published: Mar 31, 2014
MONMOUTH JUNCTION, N.J. -- Authors Pan et al published an article in the journal Theranostics titled, Anti-Angiogenesis Therapy in the Vx2 Rabbit Cancer Model with a Lipase-cleavable Sn 2 Taxane Phospholipid Prodrug using avß3-Targeted Theranostic Nanoparticles’, describing nanoparticle formulations for anti-cancer chemotherapy. Research involved developing and characterizing a Sn 2 lipase-labile prodrug of docetaxel Dxtl-PD. Excessive serum and lipase surrounding taxane prodrug in PFC nanoparticles was optimized using Cleanascite™, a lipid removal reagent. In vitro experiment on anti-angiogenic molecule docetaxel prodrug in perfluorocarbon (PFC) nanoparticles cites Cleanascite™ for lipid removal from plasma-enzyme-nanoparticle mixture. Excessive lipids in samples interferes in high performance liquid chromatography. Subsequent to sample pretreatment by implementing the Cleanascite™ protocol, the mixture is centrifuged and supernatant is analyzed by HPLC. Less matrix effects are observed in high performance liquid chromatography analysis as Cleanascite™ removes lipids and does not bind to DNA, RNA, enzymes and proteins in samples. Next, 2-propranol and water was added to red blood cell aliquots and mixture was centrifuged. The RBC fraction is separated from PFC nanoparticles.
Characteristics of Cleanascite™ Lipid Removal Reagent and Clarification
Helps purify antibodies, recombinant proteins, nucleic acids, proteoglycans
Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
Clarifies saliva and fecal components
Very low protein binding
Does not bind to DNA, RNA, enzymes and proteins
Leaves glycoproteins, antibodies, nucleic acids, hemoglobin, proteoglycans, nucleic acids, serum components(such as hormones, nutrients, globulins, clotting factors, transport proteins) alone
Extends the life of membrane and chromatographic columns.
Enrichment of delipidated tissue samples
Ideal for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monoclonal antibodies.
Selectively removes lipids, cell debris, lipoproteins, floating fats, impurities from cohn paste, transgenic milk, egg yolk and biological samples for pretreatment of samples prior to purification.
For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification
About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuSorb™& AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ & ProPrep™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com
Dr. Swapan Roy
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
800-935-0628 North America
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