Biotech Support Group Release: Enrichment of DNA From Protein Samples Using ProCipitate™
Published: Aug 20, 2012
Preparation of large quantity and high quality genomic DNA requires gathering cells containing DNA, releasing DNA from collected cells, separation of DNA from proteins and cellular contaminants and isolation of concentrated DNA for use in genetic and genomic analyses techniques such as genetic mapping, TILLING (Targeting Induced Local Lesion IN Genome), and next-generation sequencing. ProCipitate™ is high throughput, high yield, and cost efficient for small or large sample genomic DNA enrichment. It is compatible with high throughput genomic DNA isolation and produces high quality, improved yield of DNA over alternative bind and elute systems. ProCipitate™ is available as a suspension reagent and in ProPrep™ kits for specific applications and high-throughput 96 well filter formats.
For more information click: http://www.biotechsupportgroup.com/node/114
About Biotech Support Group LLC Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com.
Biotech Support Group
1 Deer Park Drive, Suite M,
Monmouth Junction, NJ 08852, USA
Kozekov ID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ. Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Chemical Research in Toxicology.2010;23(11):1701-1713
Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman. Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts BMC Genomics.2006;7: 301
Krupey, J., et al, 100,000+ PCRs Possible from 10 ml Blood, poster Biotechniques Symposium, 2003.
Quiniou SM; Katagiri T; Miller NW; Wilson M; Wolters WR; Waldbieser GC Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Genetics, selection, evolution : GSE. 2003;35(6):673-83
J M Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; M D Adams. High Throughput Direct End Sequencing of BAC Clones. Nucleic Acids Research.1999.15;27(6):1539-1546
DNA Extraction Genetic Diversity as an Indicator of Ecosystem Condition and Sustainability for Regional Assessments of Stream Condition in the Eastern United States
David C. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L. Robinson; Norman A. Doggett; Larry L. Deaven. BAC Library End Sequencing in Support of Whole Genome Assemblies. DOE Joint Genome Institute and Center for Human Genome Studies, Los Alamos National Laboratory
G.M. Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood A High-Throughput Plasmid DNA Preparation Method Analytical Biochemtry.1994;15;223(1):35-8
Robert R. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; John E. Mullet. High Throughput BAC DNA Isolation for Physical Map Construction of Sorghum. Plant Molecular Biology Reporter.1988;16(4):651-364