Bio-Rad Laboratories, Inc.'s New ddPCR Library Quantification Kit Optimizes Performance of Ion Torrent NGS Systems
Published: Sep 24, 2013
Hercules, CA — September 24, 2013 — Bio-Rad Laboratories, Inc., today announced the availability of its new ddPCR™ library quantification kit for Ion Torrent library preparation. Used with Bio-Rad’s QX200™ Droplet Digital™ PCR system, the new kit provides researchers with the ability to precisely and directly measure amplifiable library concentrations.
The Ion AmpliSeq library kit is used to prepare libraries for Ion Torrent next-generation sequencing (NGS) systems. Using the ddPCR library quantification kit to quantify Ion AmpliSeq gDNA and RNA libraries maximizes the number of useable reads, enables consistent loading, and optimizes the utilization of every sequencing run. The resulting data provide additional measures of library quality not provided by other methods, including the percentage of nonamplifiable species such as adapter dimers and the size range of library inserts.
Additional key benefits of the ddPCR library quantification kit for Ion Torrent systems include:
- Superior performance — produces highly precise measurements of amplifiable library concentrations without the use of standards
- Visualization of library quality — ddPCR fluorescence amplitude plots highlight well formed and poorly formed libraries
- Efficient utilization of sequencing runs — enables consistent loading and maximum efficiency of the Ion Torrent sequencing platforms
Kits for other NGS platforms are also in development. For more information on the ddPCR library quantification kit, please visit: www.bio-rad.com/ion-torrent.
Ion Torrent and Ion AmpliSeq are trademarks of Life Technologies Corporation.
Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb) has been at the center of scientific discovery for 60 years, manufacturing and distributing a broad range of products for life science research and clinical diagnostic markets. The company is renowned for its commitment to quality and customer service among university and research institutions, hospitals, public health and commercial laboratories, as well as the biotechnology, pharmaceutical, and food safety industries. Founded in 1952, Bio-Rad is based in Hercules, California, and serves more than 100,000 research and industry customers through its global network of operations. The company employs approximately 7,600 people worldwide and had revenues exceeding $2 billion in 2012. Visit us at www.bio-rad.com.
For more information contact:
Bio-Rad Laboratories, Inc.
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