Integrated DNA Technologies Release: Identifying Epigenetic Biomarkers For Prostate Cancer
3/31/2014 10:07:43 AM
CORALVILLE, IA – 31 March 2014 – In the latest issue of DECODED, Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, talks about how the prognosis for men with aggressive forms of prostate cancer is improved if detected early. Dr Antoinette Perry, Senior Research Fellow at the Institute of Molecular Medicine (Dublin, Ireland) leads a team whose research is directed at understanding how changes in DNA methylation of both coding and non-coding RNA genes are involved in driving prostate carcinogenesis.
Previously published work by Dr Perry revealed that SFRP2, a Wnt signalling agonist, is hypermethylated in approximately 65% of prostate tumors. IDT PrimeTime® qPCR Assays were used to measure expression levels of SFRP2, and subsequent analysis showed an inverse correlation between expression and tumor grade. As part of a further investigation into the involvement of hypermethylated genes in prostate cancer, prognostic markers are being developed for use in a non-invasive test that will distinguish high-risk from lower-risk tumors. Following cell recovery, IDT primers and ZEN™ Double-Quenched Probes are used for sensitive quantification of prostate cancer-specific DNA methylation. In addition to the cellular component, the scientists monitor cell-free nucleic acids for DNA methylation and expression of small non-coding RNAs. The IDT PrimerQuest (idtdna.com/scitools) is being used to design methylation-specific assays (primers and probes that will hybridize specifically to bisulfite modified methylation target regions, but that will not amplify unconverted genomic DNA or unmethylated DNA).
Following a recommendation from a colleague, IDT now supplies products into several of the Perry laboratory projects. For more information please visit www.idtdna.com. Follow us on twitter @idtdna for real-time updates and insights.
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the world, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; Leuven, Belgium; and Singapore. For more information visit www.idtdna.com.
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