ROCKFORD, Ill. (January 30, 2012) – Thermo Fisher Scientific, the world leader in serving science, today introduced two new kits that enable the in vitro expression of full-length proteins from mRNA or DNA templates with yields of up to 750 micrograms per milliliter of reaction. The expressed proteins by either the Thermo Scientific 1-Step Human Coupled IVT Kit for DNA or the 1-Step Human IVT Kit for RNA can be used to study protein interactions, to perform mutational analysis or for structural studies.
The 1-Step Human IVT Kits rely on the unique HeLa cell lysate that contains all the machinery for in vitro protein expression. While 1-Step Human Coupled IVT reactions are complete in 90 minutes, reactions can be extended for up to six hours for higher protein yields. These kits can produce proteins greater than 250 kilodaltons in size and can express functional ezymes, phosphoproteins, glycoproteins and membrane proteins.
In addition to the 1-Step Human IVT Kits, 10 new expression vectors optimized for use with these lysates are now available. The vectors offer the choice of different protein purification tags. All vectors contain the EncephaloMyoCarditis Virus (EMCV) Internal Ribosome Entry Site (IRES) that is critical for high-level translation of mRNAs. A custom service to clone target sequences into these vectors is also available.
For more information on the 1-Step Human IVT Kits and new vectors, please visit www.thermoscientific.com/pierce.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of nearly $11 billion, we have approximately 37,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through two premier brands, Thermo Scientific and Fisher Scientific, which offer a unique combination of continuous technology development and the most convenient purchasing options. Our products and services help accelerate the pace of scientific discovery, and solve analytical challenges ranging from complex research to routine testing to field applications. Visit www.thermofisher.com.