eNewsletter Signup
Miles
Km80.5

Staff Scientist, Membrane Protein Chemistry

Takeda California

Apply
E-Mail to a Friend Add to Job Folder View All Jobs Back to Search Results Share
Location: San Diego, CA Posted Date: 4/10/2013 Position Type: Full time Job Code: 210 Required Education: Bachelors Degree Areas of Expertise Desired: Biochemistry
Biology
Protein Chemistry

Description

OBJECTIVES:

With minimal guidance, purify, characterize and assist crystallization of integral membrane proteins including GPCRs to support structure based drug design or assay development and compound screening for drug discovery programs.

ACCOUNTABILITIES:

•   Experimentally establishes purification schemes and performs
     hands-on small-scale and large-scale purification to obtain highly
     purified membrane proteins for crystallization in a timely manner as
     well as to support assay development and compound screening for drug
     discovery group.  
•   Develops and incorporates biochemical assays to measure stability
     and functionality of purified membrane proteins such as (but not
     limited to) protein/detergent/lipid characterization, ligand binding
     and enzymatic assays.
•   Actively supports crystallization of membrane proteins using LCP
     and vapor diffusion techniques.
•   Tests and optimizes expression conditions.
•   Develops and performs hands-on ligand binding assay to
     characterize and screen suitable constructs.
•   Provides input and feedback to molecular biologists, protein
     chemists and crystallographers in structural biology group to
     improve membrane protein expression and purification,
     crystallizability and diffraction quality of protein crystals.

Requirements

EDUCATION, EXPERIENCE, KNOWLEDGE AND SKILLS:

•   Ph.D. in life science with post doctorate and minimum 3-5 years of
     relevant experience in membrane protein chemistry for structural
     biology studies, OR
•   MS in life science with minimum 8+ years relevant experience OR  
•   BS in life science with minimum 14+ years of relevant experience
•   Must have strong hands-on experience and method development skills
     in membrane protein expression, purification and characterization,
     experience in membrane protein X-ray crystallography is a plus.
•   Proven track record of knowledge and expertise in membrane protein
     chemistry demonstrated through principal authorship in peer-reviewed
     publication(s).
•   Demonstrated independent thought, scientific creativity, problem-
     solving capability and able to convey ideas in a scientifically
     concise manner.
•   Excellent verbal and written communication skills as well as
     organizational skills are essential, must be able to work in a team
     environment.

PHYSICAL DEMANDS:

•   Prolonged standing and/or sitting
•   Manual dexterity required to operate office and heavy lab
     equipment    
•   Carrying, handling and reaching for objects
•   Working in cold room (4°C)
•   Exposure to hazardous/toxic/dangerous chemicals

TRAVEL REQUIREMENTS:

•   May travel to various domestic conferences or client sites,
     including overnight trips. Some international travel may be required.
•   A minimum of 2% travel.

We are an equal opportunity employer.
Apply
E-Mail to a Friend Add to Job Folder



By applying to/saving a job using , you are agreeing to comply with and be subject to the Terms of Use for use of our website. To use our website, you must agree with the Terms of Use and both meet and comply with their provisions.

ADVERTISEMENTS