This position develops, executes, and interprets in vitro and/or ex vivo immune cell functional and signaling assays using primary human, cynomolgus monkey, and/or mouse cells and cell lines to support translational efforts and bridging discovery and development projects. This role will also contribute the identification and validation of biomarker assays to support clinical programs. Specific activities include:
• Independently design, optimize, execute and interpret qPCR, ELISA and flow cytometry assays in support of antibody programs, contributing to program strategies and success:
o in vitro mechanism of action studies; cellular functional and signaling assays using primary mouse, cyno or human cells and cell lines to evaluate cellular activity of candidate antibodies against specific targets
o identification of predictive and/or pharmacodynamics biomarkers for preclinical studies.
• Execute biomarker experiments in line with one or more project strategies and, in collaboration with Discovery and Clinical development team members
• Actively search literature to identify alternative methodologies and solve scientific problems, validating and establishing, as appropriate.
• Organize, summarize and present data and analysis to supervisor, project leader, and at project and department meetings.
• Interact as needed to coordinate hand-off of materials between departments.
• Develop an expanding technical and theoretical knowledge base in project-related biology reflecting a growing understanding of the scientific literature
• Prepare technical reports, summaries, and detailed protocols
• Proactively contributes to projects by problem solving, mentoring junior colleagues, assisting colleagues with troubleshooting, and implementing new procedures, while maintaining quality of own work.
• BS/BA in a biology-related discipline and 8+ years of related laboratory experience or MS/MA with 6+ years of related laboratory research experience; including a strong working experience with therapeutic antibodies
• Strong understanding of and background in cellular immunology.
• In-depth technical and theoretical knowledge, and practical experience in, principles and practices of cell biology methodologies and cellular immunology, including mammalian cell culture, cell transfection, functional/in-vitro cellular immunology assays and cellular assay development.
• Expertise in developing and executing cellular signaling assays including qPCR, ELISA, luminescence, and flow cytometry-based readouts.
• Extensive experience in primary mouse, cyno, or human lymphocyte and mononuclear cell purification and functional assays.
• Able to apply literature to problem solving.
• Able to learn new laboratory techniques efficiently, troubleshoot experiments, and ensure experiments are well designed and performed to a high standard.
• Ability to maintain productivity and quality results for routine, established assays, while working on multiple projects, tasks.
• Ability to work on complex problems where analysis requires an in-depth evaluation of multiple factors. Exercises judgment in selecting experimental methods, techniques, evaluation criteria for obtaining results, and data analysis.
• Networks with key contacts outside own area of expertise.
• Ability to communicate clearly, and to report and present experimental results and analysis to supervisor, department colleagues, and at project team meetings.