DNS is seeking an experienced protein chemist for the structural biology department. The candidate will be responsible for the development and implementation of protein purification processes for macromolecular crystallography studies as well as for biochemical/biophysical assay development and screening. The candidate is also required to have an extensive technical background in state-of-the-art protein purification equipment such as AKTA and processes necessary to establish purification procedures that reproducibly lead to the generation of crystallization-grade protein in quantity and quality suitable for structural studies. Major responsibilities will include independent hands-on experimental protein chemistry work to support large scale protein purification and characterization. Experience in membrane protein chemistry and/or protein crystallography is a plus.
The candidate will be working in a creative, dynamic environment and will be required to be team-oriented with a desire to proactively seek out and implement new technologies to improve construct design, expression, purification and crystallization of proteins for 3D structural studies. The candidate must have excellent interpersonal skills and must be organized and timely with regard to data generation, meeting presentations and study reports.
Ph.D. in life science with thesis.
• 2+ years of relevant experience in protein structural biology
• Expert knowledge and hands-on experience in successful expression, purification, bioanalytical and biochemical characterization of proteins originating from different protein families for crystallization and 3D-structure determination.
• Proven hands-on experience in different protein purification methods (e.g affinity, size-exclusion, ion-exchange, isoelectric focusing chromatography, etc.) and biochemical/biophysical characterization techniques (e.g., Western blot, light scattering, calorimetry, analytical ultracentrifugation, DLS, thermal melting, protein MassSpec, in vitro assay development, etc.).
• Proven in-depth knowledge and expertise in protein structural biology demonstrated preferably through principle authorship in peer-reviewed publication(s).
• Demonstrated independent thought/creativity in science. Excellent collaboration, communication (oral and written) and multitasking skills and able to present work formally to Scientific Research Committees.