Scientist I or II - Cell Line Development/AAV - Gaithersburg, MD | Biospace
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Scientist I or II - Cell Line Development/AAV

MedImmune, LLC

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Location:
Gaithersburg, MD
Posted Date:
9/24/2017
Position Type:
Full time
Job Code:
R-012378
Salary:
Required Education:
Bachelors Degree
Areas of Expertise Desired:

Job Description

Location:
Gaithersburg, Maryland, United States
Job reference: R-012378
Posted date: Aug. 11, 2017

If you’re inspired by the possibilities of science to change lives and you thrive when making brave decisions – join us.

MedImmune is the worldwide biologics research and development arm of AstraZeneca. Here, you’ll have the opportunity to make a difference in people’s lives every day. As one of the world’s premier biotechnology companies, our mission is centered on delivering life-changing products that advance world health, and help fight and cure disease.  As a Scientist I or II in the Cell Line Development group in Gaithersburg, MD you’ll play a pivotal role in channeling our scientific capabilities to make a positive impact on changing patients’ lives.

We’re constantly pushing the boundaries of science to deliver life-changing medicines to patients, with a passion for discovery and a pipeline to show for it. We’re pioneering innovative research and exploring novel pathways across key therapeutic areas including oncology, respiratory, inflammation and autoimmunity, cardiovascular and metabolic disease, and infection and vaccines. And we’re industry-leading in immunology, protein engineering and translational science. We offer a unique and strong collaborative network as part of the AstraZeneca family, as together we explore synergies between small and large molecules.

MedImmune has a dynamic environment that fosters collaboration and innovation. We attract top minds, and we nurture and build top talent. Our search for the Scientist I or II is focused on candidates who possess strong molecular biology and AAV experience.

Responsibilities include:

  • Leading stable cell line development projects to deliver recombinant CHO lines for clinical supply of therapeutic proteins including: expression vector design and construction; cell transfection, sub-culturing and screening of recombinant cell lines; and molecular characterisation of candidate production cell lines.
  • Leading the development, evaluation and implementation of technologies and processes that improve efficiency, performance and capabilities of stable cell line development platforms.
  • Coordination with cross-functional teams across MedImmune to achieve project goals.
  • Keeping line manager and team informed of project progress.
  • Maintaining accurate and up-to-date electronic laboratory notebooks according to MedImmune best practice.
  • Authoring, reviewing and contributing to written reports, procedures and presentations as required.
  • Supporting the Cell Line Development and Engineering team goals and facilities.
  • Implementation of MedImmune’s health and safety practices.

Essential Qualifications

  • Master's degree in biological sciences with 10+ years of experience; PhD strongly preferred
  • Molecular biology relating to expression vector construction and molecular characterization of recombinant cell lines; including vector design, DNA cloning and sequence analysis, Q-PCR for expression analysis, cDNA sequencing, western blotting and flow cytometry.
  • Engineering of stable mammalian cell lines for recombinant protein production; including DNA transfection, cell line selection, screening, subculture and cryopreservation.

Desirable Qualifications

Practical knowledge and experience of any of the following areas:

  • AAV engineering and production
  • Generation of recombinant cell lines suitable for biopharmaceutical manufacture, including using high throughput screening systems such as FACS, cell culture automation or microbioreactor platforms.
  • Systems biology including transcriptome and pathway analysis.
  • Genome editing technologies, such as CRISPR, CRE-LoxP or integrases

Knowledge/skills/behavioral competencies

Essential:

  • Proactive communicator
  • Good organization and planning skills
  • Strong team player
  • Able to operate independently with minimal supervision
  • Able to troubleshoot
  • High level of attention to detail
  • Good presentational skills
  • Ability to work in multidisciplinary teams

Any Special Requirements

  • Flexibility to perform laboratory work at weekend and on holidays by agreement as required

Requirements

Please See Description