DNS is seeking an energetic and highly motivated Research Associate to join the protein production group within the Structural Biology department. The candidate will work as part of a team of scientists to support the recombinant production of proteins in milligram-scale quantities for macromolecular crystallographic studies. Major responsibilities will include protein expression in E-coli, insect cell-baculovirus and mammalian cell systems; maintenance of cell lines and cell culture facility; and high throughput expression screening and small/midi scale protein purification. The candidate will be working in a creative, dynamic environment and will be required to work with limited guidance in a team-oriented manner. The candidate is required to closely interact with molecular biologists and protein chemists in the group and must have strong interpersonal skills.
• A BS or MS in life science, with 2+ years of relevant experience in recombinant protein expression, preferably in an industrial setting.
• Proven hands-on experience in recombinant protein expression in prokaryotic (E-coli) and Eukaryotic (baculovirus/mammalian) expression systems. Hands-on experience in insect cell culture and transfection. Experience in biophysical/biochemical protein characterization (Western blot, SDS-PAGE, MassSpec and chromatography).
• Knowledge of principles of recombinant protein expression in prokaryotic and eukaryotic systems. Knowledge of fundamentals in protein chemistry and biophysical/biochemical protein characterization. Background knowledge in structural biology or membrane protein science is an advantage.
• Demonstrated independent thought/creativity in science. Excellent collaboration, organizational, communication (oral and written) and multitasking skills.
• Must be willing to work flexible hours and to meet project timelines.