Drug Discovery Scientist I-1306895
This lab is a multi-disciplinary group in the Department of Biochemistry at Vanderbilt University, dedicated to oncology drug discovery. We have an exciting opportunity for a talented and motivated cell biologist to join this dynamic group. This individual will work closely with students, post-docs, biologists, and chemists to screen compounds in cell-based assays, develop new assays and techniques, and contribute to the discovery of new targets for cancer drug discovery.
Key Functions and Expected Performances:
· Conduct cell-based and plate-based assays to enable drug discovery efforts. Use established protocols and existing assays to measure growth inhibition, apoptosis, regulation of transcription, and inhibition of signaling pathways; assays include fluorescence- and luminescence-based formats, such as ELISA, In-cell Western, and FRET. Screen compounds in single concentration and dose-response schemes, analyze and report data.
· Contribute to the design and development of novel assays to measure the functional consequences of inhibition of a number of diverse targets, including assays to measure disruption of protein-protein interactions, anchorage-independent growth, migration, invasion, and signaling.
· Conduct research projects to understand the mechanism of action of novel targeted therapies identified in our group. This may include screening cancer cell lines and tumors to understand the genetic predictors of response to these targeted agents.
· Suggest, design, and implement research projects to identify and validate new targets for cancer drug discovery. Experiments may include target silencing by RNAi, target overexpression, and compound treatment of cancer cell lines.
· Remain current on literature, ideas, and relevant laboratory techniques in oncology and drug discovery.
· Contribute to the effective operation of the lab by sharing the responsibilities of preparing buffers and reagents, ordering supplies, and maintaining equipment.
The ideal candidate would have a Ph.D. in cell or cancer biology or a Masters degree with a minimum of 5 years experience in oncology/cancer biology.
The candidate must be well-versed in standard cell biology techniques, including mammalian cell culture, transfection protocols, Western blotting techniques, fluorescence microscopy, and PCR. Candidate must be able to design and troubleshoot assays and experiments independently. Clear written and oral communication skills, excellent data analysis capabilities, and proficiency with curve-fitting and graphing programs are essential. Applicants with previous compound screening or drug discovery experience in a pharmaceutical or biotech company are highly desired.
Job requires Bachelor's and 3 years of experience or the equivalent.
Biochemistry Sch 104250