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PLoS By Category | Recent PLoS Articles
Molecular Biology - Physiology - Respiratory Medicine - Biochemistry

Identification of Transcription Factors Regulating CTNNAL1 Expression in Human Bronchial Epithelial Cells
Published: Thursday, February 16, 2012
Author: Yang Xiang et al.

by Yang Xiang, Xiao-Qun Qin, Hui-Jun Liu, Yu-Rong Tan, Chi Liu, Cai-Xia Liu

Adhesion molecules play important roles in airway hyperresponsiveness or airway inflammation. Our previous study indicated catenin alpha-like 1 (CTNNAL1), an alpha-catenin-related protein, was downregulated in asthma patients and animal model. In this study, we observed that the expression of CTNNAL1 was increased in lung tissue of the ozone-stressed Balb/c mice model and in acute ozone stressed human bronchial epithelial cells (HBEC). In order to identify the possible DNA-binding proteins regulating the transcription of CTNNAL1 gene in HBEC, we designed 8 oligo- nucleotide probes corresponding to various regions of the CTNNAL1 promoter in electrophoretic mobility shift assays (EMSA). We detected 5 putative transcription factors binding sites within CTNNAL1 promoter region that can recruit LEF-1, AP-2a and CREB respectively by EMSA and antibody supershift assay. Chromatin immunoprecipitation (ChIP) assay verified that AP-2 a and LEF-1 could be recruited to the CTNNAL1 promoter. Therefore we further analyzed the functions of putative AP-2 and LEF-1 sites within CTNNAL1 promoter by site-directed mutagenesis of those sites within pGL3/FR/luc. We observed a reduction in human CTNNAL1 promoter activity of mutants of both AP-2a and LEF-1 sites. Pre-treatment with ASOs targeting LEF-1and AP-2a yielded significant reduction of ozone-stress-induced CTNNAL1 expression. The activation of AP-2a and LEF-1, followed by CTNNAL1 expression, showed a correlation during a 16-hour time course. Our data suggest that a robust transcriptional CTNNAL1 up-regulation occurs during acute ozone-induced stress and is mediated at least in part by ozone-induced recruitments of LEF-1 and AP-2a to the human CTNNAL1 promoter.
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