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PLoS By Category | Recent PLoS Articles
Immunology - Oncology - Physics - Radiology and Medical Imaging

X-ray Phase Contrast Imaging of Cell Isolation with Super-Paramagnetic Microbeads
Published: Monday, September 24, 2012
Author: Rongbiao Tang et al.

by Rongbiao Tang, Wei-Min Chai, Guo-Yuan Yang, Honglan Xie, Ke-Min Chen

Super-paramagnetic microbeads are widely used for cell isolation. Evaluation of the binding affinity of microbeads to cells using optical microscopy has been limited by its small scope. Here, magnetic property of microbeads was first investigated by using synchrotron radiation (SR) in-line x-ray phase contrast imaging (PCI). The cell line mouse LLC (Lewis lung carcinoma) was selected for cell adhesion studies. Targeted microbeads were prepared by attaching anti-VEGFR2 (vascular endothelial growth factor receptor-2) antibody to the shell of the microbeads. The bound microbeads were found to better adhere to LLC cells than unbound ones. PCI dynamically and clearly showed the magnetization and demagnetization of microbeads in PE-50 tube. The cells incubated with different types of microbeads were imaged by PCI, which provided clear and real-time visualization of the cell isolation. Therefore, PCI might be considered as a novel and efficient tool for further cell isolation studies.
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