BioSpace Collaborative

Academic/Biomedical Research
News & Jobs
Biotechnology and Pharmaceutical Channel Medical Device and Diagnostics Channel Clinical Research Channel BioSpace Collaborative    Job Seekers:  Register | Login          Employers:  Register | Login  

NEWSLETTERS
Free Newsletters
Archive
My Subscriptions

NEWS
News by Subject
News by Disease
News by Date
PLoS
Search News
Post Your News
JoVE

CAREER NETWORK
Job Seeker Login
Most Recent Jobs
Search Jobs
Post Resume
Career Fairs
Career Resources
For Employers

HOTBEDS
Regional News
US & Canada
  Biotech Bay
  Biotech Beach
  Genetown
  Pharm Country
  BioCapital
  BioMidwest
  Bio NC
  BioForest
  Southern Pharm
  BioCanada East
  US Device
Europe
Asia

DIVERSITY

PROFILES
Company Profiles

INTELLIGENCE
Research Store

INDUSTRY EVENTS
Research Events
Post an Event
RESOURCES
Real Estate
Business Opportunities

PLoS By Category | Recent PLoS Articles
Infectious Diseases - Microbiology - Public Health and Epidemiology - Urology

Multi-Locus Variable Number of Tandem Repeat Analysis for Rapid and Accurate Typing of Virulent Multidrug Resistant Escherichia coli Clones
Published: Friday, July 27, 2012
Author: Umaer Naseer et al.

by Umaer Naseer, Barbro E. Olsson-Liljequist, Neil Woodford, Hiran Dhanji, Rafael Cantón, Arnfinn Sundsfjord, Bjørn-Arne Lindstedt

One hundred E. coli isolates from Norway (n?=?37), Sweden (n?=?24), UK (n?=?20) and Spain (n?=?19), producing CTX-M-type - (n?=?84), or SHV-12 (n?=?4) extended spectrum ß-lactamases, or the plasmid mediated AmpC, CMY-2 (n?=?12), were typed using multi-locus sequence typing (MLST) and multi-locus variable number of tandem repeat analysis (MLVA). Isolates clustered into 33 Sequence Types (STs) and 14 Sequence Type Complexes (STCs), and 58 MLVA-Types (MTs) and 25 different MLVA-Type Complexes (MTCs). A strong agreement between the MLST profile and MLVA typing results was observed, in which all ST131-isolates (n?=?39) and most of the STC-648 (n?=?10), STC-38 (n?=?9), STC-10 (n?=?9), STC-405 (n?=?8) and STC-23 (n?=?6) isolates were clustered distinctly into MTC-29, -36, -20, -14, -10 and -39, respectively. MLVA is a rapid and accurate tool for genotyping isolates of globally disseminated virulent multidrug resistant E. coli lineages, including ST131.
  More...

 

//-->