|
Academic/Biomedical Research
News & Jobs
|
|
|
|
|
|
|
|
|
|
|
|
|
Free Newsletters
Archive
My Subscriptions
News by Subject
News by Disease
News by Date
PLoS
Search News
Post Your News
JoVE
Job Seeker Login
Most Recent Jobs
Search Jobs
Post Resume
Career Fairs
Career Resources
For Employers
Regional News
US & Canada
Biotech Bay
Biotech Beach
Genetown
Pharm Country
BioCapital
BioMidwest
Bio NC
BioForest
Southern Pharm
BioCanada East
US Device
Europe
Asia
Company Profiles
Research Store
Research Events
Post an Event
Real Estate
Business Opportunities
|
|
|
|
PLoS By Category | Recent
PLoS Articles
|
|
Biochemistry - Nephrology
|
Functional Consequences of Cell Type-Restricted Expression of Laminin a5 in Mouse Placental Labyrinth and Kidney Glomerular Capillaries
Published:
Friday, July 20, 2012
Author:
Sung Tae Kim et al.
by Sung Tae Kim, Tracy L. Adair-Kirk, Robert M. Senior, Jeffrey H. Miner
The labyrinth is the highly vascularized part of the rodent placenta that allows efficient transfer of gases, nutrients, wastes, and other molecules between the maternal and embryonic circulations. These two blood compartments are separated by blastocyst-derived trophoblasts and endothelial cells with an intervening basement membrane that contains laminin and other typical basement membrane components. Previously we reported that the labyrinth of laminin a5 knockout (LMa5-/-) embryos exhibits reduced vascularization and detachment of endothelial cells from the basement membrane, which normally contains LMa5. As very little is known about the origin of this vascular basement membrane, we investigated the cellular requirements for LMa5 expression in the mouse placental labyrinth. By fluorescence-activated cell sorting and RT-PCR we confirmed that both endothelial cells and trophoblasts normally express LMa5. Using Cre-loxP technology and doxycycline-mediated gene expression, we generated genetically mosaic placentas in which either the trophoblasts or the endothelial cells, but not both, expressed LMa5. We found that the overall architecture of the labyrinth was normal as long as one of these two cell types expressed LMa5, even if it was transgene-derived human laminin a5. These results suggest that laminin trimers containing a5 that are synthesized and secreted by endothelium or by trophoblasts are capable of integrating into the basement membrane and promoting normal vascularization of the placenta. Additional studies showed that endothelium-expressed human LMa5 can support vascularization of the kidney glomerulus, consistent with previous studies using a tissue grafting approach.
More...
|
|
|
 |
|
|
|
|
|
|
|
|