BioSpace Collaborative - Simultaneous Detection of Ca2+ and Diacylglycerol Signaling in Living Cells

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PLoS By Category | Recent PLoS Articles

Biophysics - Biotechnology - Neuroscience - Physiology

Simultaneous Detection of Ca2+ and Diacylglycerol Signaling in Living Cells
Published: Friday, August 17, 2012
Author: Paul Tewson et al.

by Paul Tewson, Mara Westenberg, Yongxin Zhao, Robert E. Campbell, Anne Marie Quinn, Thomas E. Hughes

Phospholipase C produces two second messengers - diacylglycerol (DAG), which remains in the membrane, and inositol triphosphate (IP₃), which triggers the release of calcium ions (Ca²⁺) from intracellular stores. Genetically encoded sensors based on a single circularly permuted fluorescent protein (FP) are robust tools for studying intracellular Ca²⁺ dynamics. We have developed a robust sensor for DAG based on a circularly permuted green FP that can be co-imaged with the red fluorescent Ca²⁺ sensor R-GECO for simultaneous measurement of both second messengers. More...