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PLoS By Category | Recent
PLoS Articles
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Biochemistry
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SCaMC-1Like a Member of the Mitochondrial Carrier (MC) Family Preferentially Expressed in Testis and Localized in Mitochondria and Chromatoid Body
Published:
Friday, July 06, 2012
Author:
Ignacio Amigo et al.
by Ignacio Amigo, Javier Traba, Jorgina Satrústegui, Araceli del Arco
Mitochondrial carriers (MC) form a highly conserved family involved in
solute transport across the inner mitochondrial membrane in eukaryotes. In
mammals, ATP-Mg/Pi carriers, SCaMCs, form the most complex subgroup with four
paralogs, SCaMC-1, -2, -3 and -3L, and several splicing variants. Here, we
report the tissue distribution and subcellular localization of a mammalian-specific
SCaMC paralog, 4930443G12Rik/SCaMC-1Like (SCaMC-1L),
which displays unanticipated new features. SCaMC-1L proteins show higher amino
acid substitution rates than its closest paralog SCaMC-1. In mouse, SCaMC-1L
expression is restricted to male germ cells and regulated during spermatogenesis
but unexpectedly its localization is not limited to mitochondrial structures.
In mature spermatids SCaMC-1L is detected in the mitochondrial sheath but
in previous differentiation stages appears associated to cytosolic granules
which colocalize with specific markers of the chromatoid body (CB) in post-meiotic
round spermatids and inter-mitochondrial cement (IMC) in spermatocytes. The
origin of this atypical distribution was further investigated by transient
expression in cell lines. Similarly to male germ cells, in addition to mitochondrial
and cytosolic distribution, a fraction of SCaMC-1L-expressing COS-7 cells
display cytosolic SCaMC-1L-aggregates which exhibit aggresomal-like features
as the CB. Our results indicate that different regions of SCaMC-1L hinder
its import into mitochondria and this apparently favours the formation of
cytosolic aggregates in COS-7 cells. This mechanism could be also operational
in male germ cells and explain the incorporation of SCaMC-1L into germinal
granules.
More...
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