BioSpace Collaborative

Academic/Biomedical Research
News & Jobs
Biotechnology and Pharmaceutical Channel Medical Device and Diagnostics Channel Clinical Research Channel BioSpace Collaborative    Job Seekers:  Register | Login          Employers:  Register | Login  

NEWSLETTERS
Free Newsletters
Archive
My Subscriptions

NEWS
News by Subject
News by Disease
News by Date
PLoS
Search News
Post Your News
JoVE

CAREER NETWORK
Job Seeker Login
Most Recent Jobs
Search Jobs
Post Resume
Career Fairs
Career Resources
For Employers

HOTBEDS
Regional News
US & Canada
  Biotech Bay
  Biotech Beach
  Genetown
  Pharm Country
  BioCapital
  BioMidwest
  Bio NC
  BioForest
  Southern Pharm
  BioCanada East
  US Device
Europe
Asia

DIVERSITY

PROFILES
Company Profiles

INTELLIGENCE
Research Store

INDUSTRY EVENTS
Research Events
Post an Event
RESOURCES
Real Estate
Business Opportunities

 News | News By Subject | News by Disease News By Date | Search News
Get Our Industry eNewsletter FREE email:    
   

The University of Colorado at Boulder Uses Nanoparticle Tracking Analysis to Characterize Microvesicles as Potential Biomarkers


6/19/2012 9:47:36 AM

Salisbury, UK, 19th June 2012: NanoSight, leading manufacturers of unique nanoparticle characterization technology, reports on the work of Professor Hang (Hubert) Yin's group at the University of Colorado at Boulder where they apply Nanoparticle Tracking Analysis (NTA) to characterize biological nanoparticles such as microvesicles.

The Yin Research Lab is interested in studying at the interface of chemistry, Biology and engineering with a particular focus on structure-based drug design, cell signaling, biochemistry, biotechnology development and membrane protein simulations.

The main research goal of the group is to identify and design peptides that sense membrane curvature to better understand protein/peptide-lipid interactions and potentially create non-invasive probes to detect highly curved extracellular vesicles. Currently, we are studying microvesicles as potential biomarkers of tumor progression and cancer metastasis. These nanoparticles are shed into bodily fluids targeting other cells in the body and are vital for inter-cellular communication.

Their experimental protocol involves lipid vesicle preparation by pressure-controlled extrusion through different membrane pore sizes. Different lipid vesicle sizes are prepared in order to mimic the size range of the microvesicles that are shed into the extracellular matrix. Following vesicle extrusion, it is important to validate the vesicle size. By using Nanoparticle Tracking Analysis (NTA) technology, the results provide an accurate quantification of different populations of vesicle sizes present in the sample.

Prior to NTA, the group mostly used dynamic light scattering (DLS) to determine the sizes of our synthetic lipid vesicles. Speaking on their use of NTA, Professor Yin says "NTA brought several benefits over existing methods. The detection ranges from 10 - 2000 nm for vesicle sizes, dimensions that cover our liposome size of interest. Flow cytometry has a lower limit detection of ~200 nm to accurately measure particle sizes so did not reach our lower requirement while DLS measures the average size of all the particles present in the sample rather than accurately distinguish different pools of vesicle sizes, often creating a bias towards larger particles."

The group has recently published a paper in the Journal of Visualized Experimentation that used the NTA technology entitled Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Liposomes (Leslie A. Morton, Jonel P. Saludes, Hang Yin). To learn more, please refer to doi: 10.3791/4151.

To find out about the company and to learn more about particle characterization using NanoSight's unique nanoparticle tracking analysis solutions, visit www.nanosight.com and register to receive the next issue of NanoTrail, the company's electronic newsletter.

About NanoSight:

NanoSight delivers the world's most versatile and proven multi-parameter nanoparticle analysis in a single instrument.

NanoSight's "Nanoparticle Tracking Analysis" (NTA) detects and visualizes populations of nanoparticles in liquids down to 10 nm, dependent on material, and measures the size of each particle from direct observations of diffusion. Additionally, NanoSight measures concentration and a fluorescence mode differentiates suitably-labelled particles within complex background suspensions. Zeta potential measurements assess the surface charge on particles. NTA's particle-by-particle methodology goes beyond traditional light scattering and other ensemble techniques in providing high-resolution particle size distributions and validates data with information-rich video files of the particles moving under Brownian motion.

NanoSight's simultaneous multiparameter characterization matches the demands of complex biological systems, hence its wide application in development of drug delivery systems, of viral vaccines, and in nanotoxicology. This real-time data gives insight into the kinetics of protein aggregation and other time-dependent phenomena in a qualitative and quantitative manner. NanoSight has a growing role in biodiagnostics, being proven in detection and speciation of nanovesicles (exosomes) and microvesicles.

NanoSight has installed more than 450 systems worldwide with users including BASF, GlaxoSmithKline, Merck, Novartis, Pfizer, Proctor and Gamble, Roche and Unilever together with the most eminent universities and research institutes. NanoSight's technology is validated by 400+ third party papers citing NanoSight results, consolidating NanoSight's leadership position in nanoparticle characterization. For more information, visit www.nanosight.com

For further information:

Please contact NanoSight direct or their marketing agency, Talking Science:

NanoSight Limited

Minton Park

London Road

Amesbury SP4 7RT UK

T +44(0)1980 676060

F +44(0)1980 624703

www.nanosight.com

jeremy.warren@nanosight.com

Talking Science Limited

39 de Bohun Court

Saffron Walden

Essex CB10 2BA UK

T +44(0)1799 521881

M +44(0)7843 012997

www.talking-science.com

jezz@talking-science.com



Read at BioSpace.com

   

ADD TO DEL.ICIO.US    ADD TO DIGG    ADD TO FURL    ADD TO STUMBLEUPON    ADD TO TECHNORATI FAVORITES
 

//-->