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Mettler-Toledo (MTD) Release: White Paper Explores Effective Protein Purification


10/17/2012 1:17:57 PM

Oakland, California – 11 October 2012 – Rainin Instrument, a METTLER-TOLEDO subsidiary, has published a new white paper entitled “High Throughput FPLC with Packed Resin Tip Technology.” This addition to METTLER-TOLEDO’s thought-leadership library helps shed light on how novel PureSpeedTM resin tips promote easier, faster and more cost-effective protein and antibody purification than can be achieved using traditional gravity/spin columns.

Using conventional spin columns, protein purification requires a relatively large amount of resin for acceptable performance. However, experiments detailed in the white paper indicate that the higher resin volumes required in spin columns are not the key to optimizing workflow.

PureSpeed’s bidirectional flow was shown to load PureSpeed’s relatively small resin bed with the most protein possible, providing superior final concentrations and purity when compared to the spin column procedure. Protein capture was shown to be more repeatable as well, even at significantly reduced elution volumes.

The improved concentrations that PureSpeed enables help provide samples robust enough for assaying or downstream processing. Generally, this saves time by eliminating the need to concentrate recovered protein in a separate step.

The paper also offers guidelines for using PureSpeed tip, body and bed sizes to gain maximum protein/antibody processing. For a complete copy of the paper, or for more on how PureSpeed can help streamline protein purification saving time and money in the process, visit www.mt.com/purespeed

Contact:

Terry Peckham

Global Marketing Communications Manager

(510) 564-1736

terry.peckham@rainin.com

About METTLER-TOLEDO

Rainin Instrument, LLC, is a wholly owned subsidiary of METTLER-TOLEDO, a leading supplier of precision instruments and services worldwide. Rainin pipettes have long been the largest selling brand in North America and consistently ranks among the top premium pipette brands in Europe, Asia and the Middle East. METTLER-TOLEDO is the world’s largest manufacturer and marketer of weighing instruments for use in laboratory, industrial and food retailing applications. It holds top three market positions in several related analytical instruments and is a leading provider of automated chemistry systems used in drug and chemical compound discovery and development. The company is also the world’s largest manufacturer and marketer of metal detection and other end-of-line inspection systems used in production and packaging. Additional information about Rainin and METTLER-TOLEDO can be found at www.mt.com.



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