BioSpace Collaborative

Academic/Biomedical Research
News & Jobs
Biotechnology and Pharmaceutical Channel Medical Device and Diagnostics Channel Clinical Research Channel BioSpace Collaborative    Job Seekers:  Register | Login          Employers:  Register | Login  

NEWSLETTERS
Free Newsletters
Archive
My Subscriptions

NEWS
News by Subject
News by Disease
News by Date
PLoS
Search News
Post Your News
JoVE

CAREER NETWORK
Job Seeker Login
Most Recent Jobs
Search Jobs
Post Resume
Career Fairs
Career Resources
For Employers

HOTBEDS
Regional News
US & Canada
  Biotech Bay
  Biotech Beach
  Genetown
  Pharm Country
  BioCapital
  BioMidwest
  Bio NC
  BioForest
  Southern Pharm
  BioCanada East
  C2C Services & Suppliers™
Europe
Asia

DIVERSITY

PROFILES
Company Profiles

INTELLIGENCE
Research Store

INDUSTRY EVENTS
Research Events
Post an Event
RESOURCES
Real Estate
Business Opportunities

 News | News By Subject | News by Disease News By Date | Search News
Get Our Industry eNewsletter FREE email:    
   

Clemson University Researchers Collect and Reuse Enzymes While Maintaining Bioactivity


8/8/2012 11:57:50 AM

CLEMSON — Clemson University researchers are collecting and harvesting enzymes while maintaining the enzyme’s bioactivity. Their work, a new model system that may impact cancer research, is published in the journal Small.

Enzymes are round proteins produced by living organisms that increase the rate of chemical reactions.

“We found a robust and simple way of attracting specific enzymes, concentrating them and reusing them,” said Stephen Foulger, professor in the School of Materials Science and Engineering at Clemson. “The enzymes are still functional after being harvested.”

Isolating a single type of protein from a complex mixture is the most difficult aspect of the purification process. It is vital to determine the function, structure and interactions of the protein.

The researchers baited a nanoparticle to capture and recycle an enzyme. They found a way to attach an enzyme’s target on the surface of a particle, allow the enzyme to bind to it, remove the particle and determine that the enzyme is still functional.

“We took a protein that was being produced in a soil and placed its food source on the outside of a nanoparticle and the protein essentially grabbed onto the food source,” said Foulger. “We froze the enzyme in place and removed the particle and thus found a commercially viable way to harvest these proteins."

"This baited particle approach provides a very efficient means for isolating complex enzyme systems for use in biotechnology," said Vincent Rotello, a chemistry professor at the University of Massachusetts Amherst and leading researcher in the field. "This method also provides considerable promise for biomedical applications."

The research established a universal model for concentrating and extracting known enzyme pairings, but it can be an invaluable tool in recognizing unknown ones.

“This model is foreshadowing for what we’re doing with cancer research because we’re beginning to focus on the 'outside' of nanoparticles to sequester specific proteins that direct cancer cell growth,” said Foulger.

The researchers’ goal is to alter the cellular concentration of critical proteins in cancer to disrupt the cell's ability to spread, thereby controlling its growth in the body.


Read at BioSpace.com


   

ADD TO DEL.ICIO.US    ADD TO DIGG    ADD TO FURL    ADD TO STUMBLEUPON    ADD TO TECHNORATI FAVORITES
 

//-->