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Gastroenterology and Hepatology - Infectious Diseases - Oncology - Pathology - Surgery

Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation
Published: Wednesday, October 05, 2011
Author: Jie Hu et al.

by Jie Hu, Zheng Wang, Jia Fan, Zhi Dai, Yi-Feng He, Shuang-Jian Qiu, Xiao-Wu Huang, Jian Sun, Yong-Sheng Xiao, Kang Song, Ying-Hong Shi, Qi-Man Sun, Xin-Rong Yang, Guo-Ming Shi, Lei Yu, Guo-Huan Yang, Zhen-Bin Ding, Qiang Gao, Zhao-You Tang, Jian Zhou


Recurrence prediction of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) present a great challenge because of a lack of biomarkers. Genetic variations play an important role in tumor development and metastasis.


Oligonucleotide microarrays were used to evaluate the genetic characteristics of tumor DNA in 30 HBV-related HCC patients who were underwent LT. Recurrence-related single-nucleotide polymorphism were selected, and their prognostic value was assessed and validated in two independent cohorts of HCC patients (N?=?102 and N?=?77), using pretransplant plasma circulating DNA. Prognostic significance was assessed by Kaplan-Meier survival estimates and log-rank tests. Multivariate analyses were performed to evaluate prognosis-related factors.


rs894151 and rs12438080 were significantly associated with recurrence (P?=?.003 and P?=?.004, respectively). Multivariate analyses demonstrated that the co-index of the 2 SNPs was an independent prognostic factor for recurrence (P?=?.040). Similar results were obtained in the third cohort (N?=?77). Furthermore, for HCC patients (all the 3 cohorts) exceeding Milan criteria, the co-index was a prognostic factor for recurrence and survival (P<.001 and P?=?.002, respectively).


Our study demonstrated first that genetic variations of rs894151 and rs12438080 in pretransplant plasma circulating DNA are promising prognostic markers for tumor recurrence in HCC patients undergoing LT and identify a subgroup of patients who, despite having HCC exceeding Milan criteria, have a low risk of post-transplant recurrence.