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Biochemistry - Hematology

Phosphorylation-Dependent SUMOylation of the Transcription Factor NF-E2
Published: Monday, September 10, 2012
Author: Yee-Fun Su et al.

by Yee-Fun Su, Yu-Chiau Shyu, Che-Kun James Shen, Jaulang Hwang

Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.