|
|
|
|
|
|
|
Free Newsletters
Archive
My Subscriptions
News by Subject
News by Disease
News by Date
PLoS
Search News
Post Your News
JoVE
Job Seeker Login
Most Recent Jobs
Browse Biotech Jobs
Search Jobs
Post Resume
Career Fairs
Career Resources
For Employers
Regional News
US & Canada
Biotech Bay
Biotech Beach
Genetown
Pharm Country
BioCapital
BioMidwest
Bio NC
BioForest
Southern Pharm
BioCanada East
US Device
Europe
Asia
Market Summary
News
IPOs
Company Profiles
Companies
Events
Research Store
Biotech Events
Post an Event
Real Estate
Business Opportunities
|
|
|
|
PLoS By Category | Recent
PLoS Articles
|
|
Infectious Diseases - Microbiology - Public Health and Epidemiology - Urology
|
Multi-Locus Variable Number of Tandem Repeat Analysis for Rapid and Accurate Typing of Virulent Multidrug Resistant Escherichia coli Clones
Published:
Friday, July 27, 2012
Author:
Umaer Naseer et al.
by Umaer Naseer, Barbro E. Olsson-Liljequist, Neil Woodford, Hiran Dhanji, Rafael Cantón, Arnfinn Sundsfjord, Bjørn-Arne Lindstedt
One hundred E. coli isolates from Norway (n?=?37), Sweden (n?=?24), UK (n?=?20) and Spain (n?=?19), producing CTX-M-type - (n?=?84), or SHV-12 (n?=?4) extended spectrum ß-lactamases, or the plasmid mediated AmpC, CMY-2 (n?=?12), were typed using multi-locus sequence typing (MLST) and multi-locus variable number of tandem repeat analysis (MLVA). Isolates clustered into 33 Sequence Types (STs) and 14 Sequence Type Complexes (STCs), and 58 MLVA-Types (MTs) and 25 different MLVA-Type Complexes (MTCs). A strong agreement between the MLST profile and MLVA typing results was observed, in which all ST131-isolates (n?=?39) and most of the STC-648 (n?=?10), STC-38 (n?=?9), STC-10 (n?=?9), STC-405 (n?=?8) and STC-23 (n?=?6) isolates were clustered distinctly into MTC-29, -36, -20, -14, -10 and -39, respectively. MLVA is a rapid and accurate tool for genotyping isolates of globally disseminated virulent multidrug resistant E. coli lineages, including ST131.
More...
|
|
|
 |
|
|
|
|
|
|
|
|