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Multi-Locus Variable Number of Tandem Repeat Analysis for Rapid and Accurate Typing of Virulent Multidrug Resistant Escherichia coli Clones
Published: Friday, July 27, 2012
Author: Umaer Naseer et al.

by Umaer Naseer, Barbro E. Olsson-Liljequist, Neil Woodford, Hiran Dhanji, Rafael Cantón, Arnfinn Sundsfjord, Bjørn-Arne Lindstedt

One hundred E. coli isolates from Norway (n?=?37), Sweden (n?=?24), UK (n?=?20) and Spain (n?=?19), producing CTX-M-type - (n?=?84), or SHV-12 (n?=?4) extended spectrum ß-lactamases, or the plasmid mediated AmpC, CMY-2 (n?=?12), were typed using multi-locus sequence typing (MLST) and multi-locus variable number of tandem repeat analysis (MLVA). Isolates clustered into 33 Sequence Types (STs) and 14 Sequence Type Complexes (STCs), and 58 MLVA-Types (MTs) and 25 different MLVA-Type Complexes (MTCs). A strong agreement between the MLST profile and MLVA typing results was observed, in which all ST131-isolates (n?=?39) and most of the STC-648 (n?=?10), STC-38 (n?=?9), STC-10 (n?=?9), STC-405 (n?=?8) and STC-23 (n?=?6) isolates were clustered distinctly into MTC-29, -36, -20, -14, -10 and -39, respectively. MLVA is a rapid and accurate tool for genotyping isolates of globally disseminated virulent multidrug resistant E. coli lineages, including ST131.
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