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Hematology - Infectious Diseases - Microbiology

Cryopreserved Reticulocytes Derived from Hematopoietic Stem Cells Can Be Invaded by Cryopreserved Plasmodium vivax Isolates
Published: Monday, July 23, 2012
Author: Florian Noulin et al.

by Florian Noulin, Céline Borlon, Peter van den Eede, Luc Boel, Catherine M. Verfaillie, Umberto D’Alessandro, Annette Erhart

The development of a system for the continuous culture of Plasmodium vivax in vitro would benefit from the use of reticulocytes derived from differentiated hematopoietic stem cells (HCS). At present, the need to use both fresh reticulocytes and fresh P. vivax isolates represents a major obstacle towards this goal, particularly for laboratories located in non-endemic countries. Here, we describe a new method for the cryopreservation of HSC-derived reticulocytes to be used for both P. falciparum and P. vivax invasion tests. Cryopreserved P. falciparum and P. vivax isolates could invade both fresh and cryopreserved HSC-derived reticulocytes with similar efficiency. This new technique allows the storage of HSC-derived reticulocytes which can be used for later invasion tests and represents an important step towards the establishment of a continuous P. vivax culture.