BioSpace.com

Biotech and Pharmaceutical
News & Jobs
Search the Site
 
   
Biotechnology and Pharmaceutical Channel Medical Device and Diagnostics Channel Clinical Research Channel BioSpace Collaborative    Job Seekers:  Register | Login          Employers:  Register | Login  

NEWSLETTERS
Free Newsletters
Archive
My Subscriptions

NEWS
News by Subject
News by Disease
News by Date
PLoS
Search News
Post Your News
JoVE

CAREER NETWORK
Job Seeker Login
Most Recent Jobs
Browse Biotech Jobs
Search Jobs
Post Resume
Career Fairs
Career Resources
For Employers

HOTBEDS
Regional News
US & Canada
  Biotech Bay
  Biotech Beach
  Genetown
  Pharm Country
  BioCapital
  BioMidwest
  Bio NC
  BioForest
  Southern Pharm
  BioCanada East
  US Device
Europe
Asia

DIVERSITY

INVESTOR
Market Summary
News
IPOs

PROFILES
Company Profiles

START UPS
Companies
Events

INTELLIGENCE
Research Store

INDUSTRY EVENTS
Biotech Events
Post an Event
RESOURCES
Real Estate
Business Opportunities

PLoS By Category | Recent PLoS Articles
Diabetes and Endocrinology - Immunology - Molecular Biology - Ophthalmology

IL-1ß Is Upregulated in the Diabetic Retina and Retinal Vessels: Cell-Specific Effect of High Glucose and IL-1ß Autostimulation
Published: Wednesday, May 16, 2012
Author: Yang Liu et al.

by Yang Liu, Montserrat Biarnés Costa, Chiara Gerhardinger

Many molecular and cellular abnormalities detected in the diabetic retina support a role for IL-1ß-driven neuroinflammation in the pathogenesis of diabetic retinopathy. IL-1ß is well known for its role in the induction and, through autostimulation, amplification of neuroinflammation. Upregulation of IL-1ß has been consistently detected in the diabetic retina; however, the mechanisms and cellular source of IL-1ß overexpression are poorly understood. The aim of this study was to investigate the effect of high glucose and IL-1ß itself on IL-1ß expression in microglial, macroglial (astrocytes and Müller cells) and retinal vascular endothelial cells; and to study the effect of diabetes on the expression of IL-1ß in isolated retinal vessels and on the temporal pattern of IL-1ß upregulation and glial reactivity in the retina of streptozotocin-diabetic rats. IL-1ß was quantified by RealTime RT-PCR and ELISA, glial fibrillar acidic protein, a2-macroglobulin, and ceruloplasmin by immunoblotting. We found that high glucose induced a 3-fold increase of IL-1ß expression in retinal endothelial cells but not in macroglia and microglia. IL-1ß induced its own synthesis in endothelial and macroglial cells but not in microglia. In retinal endothelial cells, the high glucose-induced IL-1ß overexpression was prevented by calphostin C, a protein kinase C inhibitor. The retinal vessels of diabetic rats showed increased IL-1ß expression as compared to non-diabetic rats. Retinal expression of IL-1ß increased early after the induction of diabetes, continued to increase with progression of the disease, and was temporally associated with upregulation of markers of glial activation. These findings point to hyperglycemia as the trigger and to the endothelium as the origin of the initial retinal upregulation of IL-1ß in diabetes; and to IL-1ß itself, via autostimulation in endothelial and macroglial cells, as the mechanism of sustained IL-1ß overexpression. Interrupting the vicious circle triggered by IL-1ß autostimulation could limit the progression of diabetic retinopathy.
  More...

 

//-->