by Monica Vasudev, Dorothy S. Cheung, Hannah Pincsak, Shun-Hwa Li, Ke Yan, Pippa Simpson, Trivikram Dasu, Mitchell H. Grayson
In a mouse model of viral induced atopic disease, expression of FceRI on dendritic cells is critical. While adult human conventional (cDC) and plasmacytoid (pDC) dendritic cells have been shown to express FceRI, it is not known if this receptor is expressed in childhood and how its expression is governed by IgE. Methods
Following informed consent of subjects (n?=?27, aged 12–188 months), peripheral blood was stained for surface expression of CD19, ILT7, CD1c, IgE, FceRI and analyzed by flow cytometry (cDC: CD19- ILT7- CD1c+; pDC: CD19- ILT7+ CD1c-). Total and specific serum IgE levels to food and inhalant allergens were determined by ImmunoCAP, and the relationship between FceRI expression on dendritic cells and sensitization, free IgE, cell bound IgE, and age was determined. Results
Independent of sensitization status, FceRI expression was noted on cDC and pDC as early as 12 months of age. Serum IgE level correlated with expression of FceRI on cDC, but not pDC. Based on the concentration of IgE, a complex relationship was found between surface bound IgE and expression of FceRI on cDC. pDC exhibited a linear relationship of FceRI expression and bound IgE that was consistent through all IgE concentrations. Conclusions
In children, FceRI expression on cDC and pDC is modulated differently by serum and cell bound IgE. IgE governance of FceRI expression on cDC depends upon a complex relationship. Further studies are needed to determine the functional roles of FceRI on cDC and pDC.