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Chemistry - Diabetes and Endocrinology - Physiology

Chronic Resveratrol Treatment Protects Pancreatic Islets against Oxidative Stress in db/db Mice
Published: Friday, November 30, 2012
Author: Young-Eun Lee et al.

by Young-Eun Lee, Ji-Won Kim, Eun-Mi Lee, Yu-Bae Ahn, Ki-Ho Song, Kun-Ho Yoon, Hyung-Wook Kim, Cheol-Whee Park, Guolian Li, Zhenqi Liu, Seung-Hyun Ko

Resveratrol (RSV) has anti-inflammatory and anti-oxidant actions which may contribute to its cardiovascular protective effects. We examined whether RSV has any beneficial effects on pancreatic islets in db/db mice, an animal model of type 2 diabetes. The db/db and db/dm mice (non-diabetic control) were treated with (db-RSV) or without RSV (db-control) (20 mg/kg daily) for 12 weeks. After performing an intraperitoneal glucose tolerance test and insulin tolerance test, mice were sacrificed, the pancreas was weighed, pancreatic ß-cell mass was quantified by point count method, and the amount of islet fibrosis was determined. 8-Hydroxydeoxyguanosine (8-OHdG), an oxidative stress marker, was determined in 24 h urine and pancreatic islets. RSV treatment significantly improved glucose tolerance at 2 hrs in db/db mice (P?=?0.036), but not in db/dm mice (P?=?0.623). This was associated with a significant increase in both pancreas weight (P?=?0.011) and ß-cell mass (P?=?0.016). Islet fibrosis was much less in RSV-treated mice (P?=?0.048). RSV treatment also decreased urinary 8-OHdG levels (P?=?0.03) and the percentage of islet nuclei that were positive for 8-OHdG immunostaining (P?=?0.019). We conclude that RSV treatment improves glucose tolerance, attenuates ß-cell loss, and reduces oxidative stress in type 2 diabetes. These findings suggest that RSV may have a therapeutic implication in the prevention and management of diabetes.