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Biophysics - Immunology - Infectious Diseases - Microbiology

Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4+ T Cells into IFN? IL10 Producing Tbet+ Th1 Cells
Published: Monday, November 12, 2012
Author: Tahsin Saygili et al.

by Tahsin Saygili, Semih Can Akincilar, Bünyamin Akgül, Ayten Nalbant

One of the heat shock family protein (Hsp) expressing bacteria is the gram negative, periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa). A. actinomycetemcomitans’ Hsp is a 64-kDa GroEL-protein, which has been shown to influence the host cells. In this study we used recombinant A. actinomycetemcomitans GroEL (rAaGroEL) protein as a model antigen to study GroEL-mediated T cell immune response. Human peripheral mononuclear cells (PBMCs), when stimulated with recombinant rAaGroEL, expressed early activation marker CD69 and IL-2R (CD25). CD25 and CD69 expressions were higher in CD4+ T cells compared to CD8+ T cells. rAaGroEL-responding CD4+ T cells expressed IL-10, IFN? and TNFa cytokines. Interestingly, there were also IL-10 and IFN? double cytokine producing CD4+ T cells. Additionally, IFN? expressing CD4+ T cells were also T-bet positive. Altogether the results suggest that rAaGroEL protein affects CD4+ T cells to differentiate into IFN? IL10-secreting T-bet+ Th1 cells.