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PLoS By Category | Recent PLoS Articles
Biotechnology - Chemistry - Microbiology - Biochemistry

Phylogenetic Diversity and Environment-Specific Distributions of Glycosyl Hydrolase Family 10 Xylanases in Geographically Distant Soils
Published: Friday, August 17, 2012
Author: Guozeng Wang et al.

by Guozeng Wang, Kun Meng, Huiying Luo, Yaru Wang, Huoqing Huang, Pengjun Shi, Peilong Yang, Zhifang Zhang, Bin Yao

Background

Xylan is one of the most abundant biopolymers on Earth. Its degradation is mediated primarily by microbial xylanase in nature. To explore the diversity and distribution patterns of xylanase genes in soils, samples of five soil types with different physicochemical characters were analyzed.

Methodology/Principal Findings

Partial xylanase genes of glycoside hydrolase (GH) family 10 were recovered following direct DNA extraction from soil, PCR amplification and cloning. Combined with our previous study, a total of 1084 gene fragments were obtained, representing 366 OTUs. More than half of the OTUs were novel (identities of <65% with known xylanases) and had no close relatives based on phylogenetic analyses. Xylanase genes from all the soil environments were mainly distributed in Bacteroidetes, Proteobacteria, Acidobacteria, Firmicutes, Actinobacteria, Dictyoglomi and some fungi. Although identical sequences were found in several sites, habitat-specific patterns appeared to be important, and geochemical factors such as pH and oxygen content significantly influenced the compositions of xylan-degrading microbial communities.

Conclusion/Significance

These results provide insight into the GH 10 xylanases in various soil environments and reveal that xylan-degrading microbial communities are environment specific with diverse and abundant populations.

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