BioSpace - FRAP Analysis: Accounting for Bleaching during Image Capture

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PLoS By Category | Recent PLoS Articles

Biochemistry - Biophysics - Biotechnology - Chemistry - Physics

FRAP Analysis: Accounting for Bleaching during Image Capture
Published: Thursday, August 09, 2012
Author: Jun Wu et al.

by Jun Wu, Nandini Shekhar, Pushkar P. Lele, Tanmay P. Lele

The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis. More...