Trevigen Launches High-Throughput PARP/Apoptosis Assays
Cell extracts can be obtained using our newly-developed, higher-throughput procedure before, during and after apoptosis induction. During apoptosis, PARP-1 which catalyzes the NAD dependent addition of poly (ADP-ribose) (PAR) onto various cytoplasmic and nuclear proteins, is cleaved from its enzymatically active form (about 116 kDa), to its inactive form (85 kDa). The HT PARP/Apoptosis Assay is an ELISA which semi-quantitatively detects PAR deposited onto immobilized histone proteins (via PARP activity) in a 96-well format.
Trevigen offers two formats of the HT PARP/Apoptosis Assay: Cat#4684-096-K (Colorimetric) and Cat# 4685-096-K (Chemiluminescent). Additional histone-coated strip wells (Cat# 4677-096-P) are available separately. For details, contact Trevigen, at 800-873-8443, info@trevigen.com or visit the website at www.trevigen.com.