Thermo Fisher Scientific Introduces Alternative to Phospho-Antibodies for the Study of Phosphotyrosine Signaling

ROCKFORD, Ill. (April 23, 2009) — Thermo Fisher Scientific, the world leader in serving science, now offers the Thermo Scientific SH2 Domain Phosphotyrosine Capture Kits, fast and efficient tools for capturing specific tyrosine phosphorylated proteins from mammalian cell lysates. These new kits enable researchers to monitor and profile specific tyrosine phosphorylation events and identify specific SH2 domain protein-protein interaction networks.

The non-antibody-based assay uses GST-tagged SH2 domain peptides and glutathione agarose resin to capture and pull down the specific binding partners, providing an alternative to traditional immunoprecipitations using phospho-antibodies. The SH2 Domain Phosphotyrosine Capture Kits also offer an alternative to phosphoprotein enrichment resins that do not discriminate among proteins that are phosphorylated on serines, threonines or tyrosines. Subsequent analysis of the SH2 domain interactions can be performed using Western blots or mass spectrometry.

Kits for studying 17 specific SH2 Domains are available (see www.thermo.com/pierce for complete listing). Each kit contains 600 ?g of a specific GST-SH2 domain and sufficient reagents (including negative controls) for six pull-down reactions with approximately 500 ?g of lysate. The kits have a shelf life of one year.

For more information on the SH2 Domain Phosphotyrosine Capture Kits, visit www.thermo.com/pierce or call +1-815-968-0747.

Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10.5 billion, we have approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. The Thermo Scientific brand represents a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com.