BioSpace.com

Biotech and Pharmaceutical
News & Jobs
Search the Site
 
   
Biotechnology and Pharmaceutical Channel Medical Device and Diagnostics Channel Clinical Research Channel BioSpace Collaborative    Job Seekers:  Register | Login          Employers:  Register | Login  

NEWSLETTERS
Free Newsletters
Archive
My Subscriptions

NEWS
News by Subject
News by Disease
News by Date
PLoS
Search News
Post Your News
JoVE

CAREER NETWORK
Job Seeker Login
Most Recent Jobs
Browse Biotech Jobs
Search Jobs
Post Resume
Career Fairs
Career Resources
For Employers

HOTBEDS
Regional News
US & Canada
  Biotech Bay
  Biotech Beach
  Genetown
  Pharm Country
  BioCapital
  BioMidwest
  Bio NC
  BioForest
  Southern Pharm
  BioCanada East
  US Device
Europe
Asia

DIVERSITY

INVESTOR
Market Summary
News
IPOs

PROFILES
Company Profiles

START UPS
Companies
Events

INTELLIGENCE
Research Store

INDUSTRY EVENTS
Biotech Events
Post an Event
RESOURCES
Real Estate
Business Opportunities

 News | News By Subject | News by Disease News By Date | Search News
eNewsletter Signup
Miles
Km80.5

   

TAP Biosystems Partners With Life Technologies (LIFE) on New Transient Expression Platform - Combined Technologies Will Offer High Throughput Automated Protein Expression


1/15/2013 11:12:34 AM

15 January 2013 -- TAP Biosystems, a leading supplier of innovative cell culture systems and consumables for life science applications, is pleased to announce a new collaboration with Life Technologies, one of the world’s foremost suppliers of cell culture products and molecular biology reagents for life science research, to develop a cost-efficient, high throughput protein expression platform.

The partnership will combine Life Technologies’ Expi293™ Expression System for transient transfection with TAP’s ambr™ advanced micro bioreactor to enable automated high throughput small scale protein expression. The increased culture consistency and productivity provided by both the Expi293 and ambr systems will offer an exciting new platform for both small volume protein expression screens and optimisation of transient expression conditions.

The ambr system uses realistic scale down bioreactors where culture conditions such as pH and DO are tightly controlled in 48 micro bioreactors (10-15 mL) in parallel, so that protein quality produced by the Expi293F cells cultured in ambr is highly consistent. The protein generated can be used with confidence in vector optimisation studies, as well as high throughput, small scale functional protein production and protein purification studies.

Dr Sanjay Vasu, Staff Scientist at Life Technologies explained: “Currently with Expi293F cells we’re seeing yields of up to 1g/L, exceeding the expression levels of even bacterial systems. The benefit of this is that scientists will have sufficient protein for analytical studies. And since this is a mammalian system, mammalian proteins are more likely to be properly folded and biologically active. We are pleased to be collaborating with TAP because using the ambr system’s automated bioprocessing capabilities; we can leverage both our platforms to provide high yields in a high throughput format for lower costs, which will represent a real breakthrough for many protein screeners.”

Dr Barney Zoro, ambr Product Manager at TAP Biosystems added: “Most high throughput protein expression is performed manually in 30mL shake flask cultures or in shaking plates, where there isn’t consistent pH and DO control. Manually intensive shake flask workflows are costly to operate, can produce variable results for productivity and protein quality, and often show poor scalability to larger bioreactor cultures. Since the Expi293 Expression System can produce milligram to gram quantities of protein with consistent quality, it is ideal to amplify this with the consistent automation and proven scalability of the ambr system. This will enable scientists to rapidly automate the generation of enough protein for analysis from 48 parallel cultures, thus saving laboratory staff time, increasing laboratory capacity and significantly reducing the cost per gram of protein produced. This powerful technology combination has the potential to revolutionise high throughput protein expression programmes and we’re delighted to be part of it.”

For further information, please contact:

Matthew Walker, Head of Communications

York Way, Royston, Hertfordshire, SG8 5WY, UK

Tel: +44 1763 227200 Fax: +44 1763 227201



Read at BioSpace.com

   

ADD TO DEL.ICIO.US    ADD TO DIGG    ADD TO FURL    ADD TO STUMBLEUPON    ADD TO TECHNORATI FAVORITES