Vancouver, BC, July 21, 2010 -- In a recent, high-impact Nature Protocol publication, Asier Sáez-Cirión and his group at the Institut Pasteur standardized the protocol for T cell-based HIV suppression. RoboSep® , the fully automated cell the fully automated cell separator from STEMCELL Technologies, was the instrument of choice for cell isolation procedures. Using RoboSep®, the group isolated CD4+ and CD8+ T cells from HIV-infected individuals for subsequent evaluation of immune correlates to determine protective CD8+ T cell response. This paper describes the detailed step-by-step procedure, along with reagent and material set up.
"This publication illustrates how RoboSep® has become the standard tool for automating cell isolation for HIV research. Since there is minimal human sample manipulation, exposure to infectious samples is significantly reduced. It is the only instrument on the market for cell isolation that employs single-use disposables, eliminating sample cross-contamination. These features make it ideal for HIV research," said Dr. Steve Woodside, Senior Scientist at STEMCELL Technologies. "More importantly, it describes how RoboSep® is advancing HIV vaccine development by providing highly purified cells for the assessment of T cell responses in HIV-infected individuals."
RoboSep® is the first instrument to offer true walk-away automation of column-free immunomagnetic cell isolation. All cell labeling and magnetic separation steps are performed with minimal sample handling, and up to four samples can be processed at one time. It is widely used in leading HIV research labs world-wide including the Laboratory of Immunoregulation at the National Institute of Allergy and Infectious Diseases (NIAID) . It was designed by STEMCELL Technologies in Vancouver, which was supported by an Industrial Research Assistance Program (IRAP) grant.
About STEMCELL Technologies
STEMCELL Technologies Inc is a leader in the development and marketing of specialty cell culture media, cell separation products and ancillary reagents for life science research. Driven by science and enabled by close interactions with global leaders in cell biology and medicine, STEMCELL delivers over 900 products to research scientists in more than 70 countries worldwide. To learn more about how STEMCELL helps to make research work, visit www.stemcell.com.