San Diego, California — January 6, 2009 — SoluLinK, Inc., a leader in conjugation products and
complete easy to use kits, announces the introduction of its newest product, the ChromaLink
One-Shot Antibody Labeling Kit. This innovative kit includes all the components for the rapid traceable
digoxigenin labeling of a single antibody. The kit features SoluLinK’s patented sulfo-ChromaLink linker, a
water-soluble, digoxigenin labeling reagent that allows rapid and direct, non-destructive quantification of
- Complete Kit: Comes complete with all necessary reagents and a foolproof protocol and reference
materials conveniently located on a flash drive in the kit.
- Built-in Quantitative Signal: Incorporated biotin is quantified using a simple UV-scan — no HABA or
Fluoro-reporter assay needed.
- Cost-Effective and Efficient: Digoxigenin labels 100 µg of a single antibody with up to 80% recovery.
- Safe and Green: Water-soluble and mild on antibodies — no DMF or other organic solvents needed.
This advanced digoxigenin labeling reagent contains an embedded bis-aryl hydrazone structure, which
forms the compound’s UV-traceable chromophore; after incorporation, digoxigenin is rapidly quantified
using a non-destructive UV-scan (220-400 nm). This traceable signature enables the nondestructive and
direct quantification of digoxigenin attached to the antibody.
Digoxigenin has long been used as an alternative hapten reporter system to the streptavidin/biotin
system. In more recent times, this reporter system has also been used side-by-side with the biotin-
streptavidin system for the detection of multiplexed antigens. Unlike the biotin reporter system,
digoxigenin incorporation has never had any known method of quantifying incorporated label like the
biotin HABA assay, until now.
The kit features high antibody recoveries (50-80%) with consistent digoxigenin incorporation. The degree
of incorporation or molar substitution ratio (MSR) ranges from 2-8 digoxigenin molecules per antibody.
This kit can be used to label a diverse group of antibodies including all mammalian IgG, IgE, and avian
ChromaLinkTM Digoxigenin One-Shot Antibody Labeling Kit is another addition to a new line of SoluLinK
products designed to address market needs for quantitative detection with effective and proven conjugation
technology, supplied in a complete, easy-to-use format. SoluLinK’s innovative bioconjugation technology
has contributed to our customers’ and collaborators’ ability to easily build greater value into their efforts and
products. With SoluLinK’s product advantages in quantification, control, stability and growing patent
portfolio, SoluLinK has established a strong leadership position in bioconjugation,” observed Leo Mendoza,
Director of Product Development at SoluLinK.
SoluLinK is the leader in the development of conjugation reagents, easy-to-use complete kits, and
conjugation services for the life science research, diagnostics, and pharmaceutical communities. These
proprietary products and services provide unique features and benefits used to link proteins,
oligonucleotides, peptides, and antibodies to each other or to a wide range of surfaces. SoluLinK’s
ChromaLinkTM Digoxigenin One-Shot Antibody Labeling Kit is just one example. This is the third in a pipeline
of new, easy-to-use labeling kits intended to enhance assay performance and measurement for customers
in a broad range of markets. SoluLinK markets its products both directly, through licensing partners and
through distributors worldwide including VWR. SoluLinK is a privately held company located in San Diego,
California. For more information, please visit www.solulink.com.
9853 Pacific Heights Blvd. Suite H,
San Diego, California 92121