Promega Corporation Introduces ONE-Glo + Tox Luciferase Reporter and Cell Viability Assays for Improved Reporter Gene Expression Analysis

August 30th 2011, Madison, WI USA: Promega Corporation announced today the launch of ONE-Glo™ + Tox Assay, for improved analysis of reporter gene expression in the context of cell health. The assay combines luciferase chemistry with a cell viability marker in a two-step, addition-only process, to enable measurement of cell viability and gene expression in a single well of a plate, negating the need to run parallel assays. By analyzing reporter activity in relation to cell viability, false results due to toxicity or changes in cell number can be uncovered. The first part of the assay is a non lytic fluorescence assay (CellTiter-Fluor™ Cell Viability Assay) that measures the relative number of the live cells in a culture population after experimental manipulation. The CellTiter-Fluor Assay measures a conserved and constitutive protease activity within live cells and therefore serves as a marker of cell viability. The substrate enters intact cells where it is cleaved by the live-cell protease to generate a fluorescent signal proportional to the number of living cells. The second part of the assay uses the ONE-Glo Lucifease Assay System to quantify firefly luciferase reporter gene expression from cells made to express this reporter enzyme. Ideally suited for high- and ultra high-throughput applications, the volumes of each assay component can be scaled to meet throughput needs and is amenable to automation up to 1,536-well format. The ONE-Glo + Tox Assay contains a new fluoroluciferin substrate, resulting in a reagent that is more stable and tolerant to sample components. Luminescence can be measured with a microplate reader or CCD imager.

For more information please visit: http://www.promega.com/oneglotox

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Enquiries: Lorna Cuddon Zyme Communications Ltd

Tel: +44 (0) 7811 996 942

Skype: lorna.cuddon

Email: lorna.cuddon@zymecommunications.com

www.zymecommunications.com

Penny Patterson Director, Corporate Affairs Promega Corporation

Phone: +1 (608) 274-4330

E-mail: penny.patterson@promega.com

www.promega.com

About Promega

Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. The company's 2,000 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA, with branches in 15 countries and over 50 global distributors.

For more information about Promega, visit: www.promega.com

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