New Stain-Free Gel Visualization Application of Syngene's G:BOX System Helps Save Time and Make Protein Analysis Safer
9/13/2013 11:06:19 AM
Frederick, MD: Scientific Digital Imaging’s [SDI’s] Syngene Division, a world-
leading manufacturer of image analysis solutions is delighted to announce that the
lighting and filter conditions in the G:BOX image analysis system have been
optimized to allow faster, safer visualization of proteins on stain-free gels.
Technical specialists at Syngene tested filter and lighting conditions in a G:BOX
XR5 image analysis system to accurately image a range of proteins (2-300ng) run
on a Criterion TGX Stain-Free gel. For optimum imaging performance, the gel was
exposed to 5 minutes of mid wave UV (302nm) on a UV transilluminator and the
gel was imaged using a UV filter and an exposure time of 2 seconds. The gel was
then stained with ProtoBlue Safe and imaged again.
The G:BOX image analysis results from the stained and unstained gel showed the
linearity and sensitivity of both methods of protein visualization was comparable
with proteins being detected in the 2-20ng range. In fact, with some proteins, the
stain- free gels provided better detection limits than the ProtoBlue Safe dye
staining. Further details of this research can be found in Application Note 64 on the Syngene web site (http://www.syngene.com/assets/doc/Technical-notes/Stainfree-gels-64.pdf).
Dr. Lindsey Kirby, SDI’s Syngene Application Specialist explained: “Staining
protein gels can be a time consuming task as scientists often use Coomassie
Blue, which is toxic and requires a lengthy de-staining step. Therefore, if scientists
can image their gels immediately after they have run them, this makes analysis
safer and more efficient.”
Lindsey added: “This is why we’ve worked to develop the optimal imaging con-
ditions for stain-free visualization. We’re delighted that our G:BOX technology is
capable of such excellent performance in this application because biologists regularly working with protein gels can now confidently use the G:BOX image analysis system for rapid stain-free visualization without compromising on sensitivity or safety.”
For Further Information Contact:
Dr Sue Pearson, Director, International Science Writer, PO Box 170, Hitchin, Hertfordshire SG5 3GD, UK.
Tel/Fax +44 (0) 1462- 635327 Email: firstname.lastname@example.org
Web: www.internationalsciencewriter.com Twitter: @isciencewriter
Syngene is a world-leading supplier of integrated imaging solutions for analysis and documentation of gel-based information. Syngene’s systems are used by more than 10,000 research organizations and over 75,000 individual scientists world-wide and include many of the world’s top pharmaceutical companies and major research institutes.
Syngene, founded in 1997, is a division of the Synoptics Group of the AIM quoted Scientific Digital Imaging Company based in Cambridge, UK. The Group’s other divisions, Syncroscopy and Synbiosis, specialize in digital imaging solutions for microscopy and microbial applications respectively. Synoptics currently employs 40 people in its UK and subsidiary operation in Frederick, USA.
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