WOBURN, Mass., Feb. 26, 2013 /PRNewswire/ -- Covaris, Inc. introduced the
single-tube FFPE DNA Extraction Kit at the 2013 Advances in Genome Biology and
Technology (AGBT) meeting held recently in Marco Island, Florida. This newest
innovation from Covaris provides an optimized reagent and protocol for
high-yield DNA extraction from Formalin-Fixed, Paraffin Embedded (FFPE) tissue
samples. Adaptive Focused Acoustic (AFA) Technology is used to emulsify and
separate the paraffin, while simultaneously re-hydrating the tissue sample in an
aqueous solution within a few minutes. Covaris Focused-ultrasonicators enable
the purely mechanical processing of the samples, eliminating the need for
organic solvents. Large Molecular Weight DNA is efficiently extracted from FFPE
samples, with greater yield when compared to organic extraction with other
commercially available kits. The AFA-based kit brings standardization to a
previously uncontrolled pre-analytical preparatory step.
The FFPE Nucleic Acid Extraction Kit utilizes AFA Technology, the standard
method for DNA fragmentation for Next Generation Sequencing (NGS). An optional
DNA fragmentation step can be included in the workflow to precisely size the DNA
for applications requiring short DNA fragments, such as NGS - all in the same
tube. This optional integrated single-tube DNA Shearing streamlines the sample
preparation workflow, while also doubling total DNA yield when compared to
extraction without fragmentation.
FFPE is a widely used method for archiving tissue specimens; especially clinical
samples. Extraction of DNA or RNA from FFPE tissue has traditionally been a
challenge as it requires removing the paraffin, rehydrating the sample, and
reversing the formaldehyde cross-links, which results in low yields and poor
quality of nucleic acid samples. Additionally, the traditional extraction
processes require the use of hazardous or difficult-to-handle solvents.
Archived FFPE tissues are a rich resource for studying gene variations or gene
expression profile changes. This trend has been growing with the continued
advances in the fields of genomics and trancriptomics. These new applications
benefit from the high-quality starting material and improved workflow provided
by the Covaris FFPE DNA Extraction Kit.
Covaris, Inc. (www.covarisinc.com) provides advanced sample preparation systems
for life and analytical science. Covaris sample prep technologies support a
wide variety of applications including Next-Generation Sequencing, ChIP,
proteomics, formulations, and compound management. Our patented Adaptive
Focused Acoustic (AFA) technology is the industry standard for DNA shearing, and
is used by major genome centers, academic, and clinical institutions worldwide.
AFA brings unsurpassed speed and efficiency to biological and chemical sample
preparation. The AFA process, which is based on shock wave physics, delivers
controlled, precise, and accurate acoustic energy to biological and chemical
SOURCE Covaris, Inc.
Brian Paras, +1-781-932-3959 x206, firstname.lastname@example.org