PRLog (Press Release) - Aug 12, 2012 - MONMOUTH JUNCTION, NJ.– UPCK™ Urine Protein Enrichment/Concentration Kit features a urinary protein enrichment optimized protocol for sample preparation and downstream analysis of urine samples using CE-MS, LC-MS, LC-MS/MS, 1-D gel analysis in combination with nano-LC MS/MS plus LTQ-FT ultra, 2-DE-MS and Multidimensional Protein Identification Technology (MudPIT) proteomic biotechniques. It is compatible with 96 well high throughput format and mild elution maintains tertiary structure and simple transfer to secondary analysis. UPCK™ Urine Protein Enrichment/Concentration Kit is ideal for mining urinary biomarkers for diabetes, diabetic nephropathy, and nondiabetic proteinuric renal diseases. Utilizing high-resolution capillary electrophoresis coupled with electrospray-ionization mass spectrometry biomarkers could be validated in blinded data sets in order to distinguish diabetic patients from healthy individuals. Researchers could identify differences in urinary biomarkers between diabetic patients having normoalbuminuria and signs of developing nephropathy. Such trends are used to identify patterns in a panel of biomarkers e.g. progression of diabetic nephropathy in patients with microalbuminuria and diabetes for early detection of disease. Urine stored in the bladder does not result in proteolytic degradation by endogenous proteases and it is ideal for protein analysis. Large volumes of urine can be processed rapidly within one hour from milliliters to liters.
Potential applications of UPCK™ Urine Protein Enrichment/Concentration Kit
Researchers Zurbig et al have published research in the journal Diabetes regarding urinary proteome analysis to predict diabetic nephropathy. Peptides and proteins in urine present from glomerular filtration, tubular secretion, epithelial cells in the kidney and urinary tract are a potential source of biomarkers for diabetic nephropathy and chronic renal disease.
Authors Theodorescu et al identified a urothelial carcinoma proteomic pattern model for predicting the presence of cancer and linked it to fibrinopeptide A-a, a biomarker of ovarian cancer and gastric cancer. Researchers also sequenced diagnostic polypeptides for urothelial carcinoma.
UPCK™ Urine Protein Concentration Kit has been especially optimized for proteomic studies of urine proteins.
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About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com.
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