PRLog (Press Release) - Apr. 11, 2013 - MONMOUTH JUNCTION, N.J. -- MONMOUTH JUNCTION, NJ.–Authors Chaumet et al published an article titled Proteomic analysis of Interleukin enhancer binding factor 3 (Ilf3) and Nuclear Factor 90 (NF90) interactome in the journal Biochimie which identified Ilf3/NF90 interacting partners by affinity chromatography. The article cites NuGel™ Poly-Amine for use in a affinity chromatography protocol with applications for immobilization of proteins & ligands via affinity purification. In this paper purified L-Ilf3 or L-NF90 proteins were immobilized onto NuGel™ Poly-Amine resin via N-(3-dimethylaminopropyl)-N0-ethyl-carbodiimide hydrochloride (EDC) coupling.
For detailed protocols of the published article click
Characteristics Of NuGel™ Poly-Amine
Non-specific sites are virtually eliminated by a polymer coating
Stable across a wide pH range 2 – 10
1000Å, 50µm Silica suitable for LC and batch processes
Applications include: Enzyme immobilization, separation and purification of biopolymers, immobilization of proteins & ligands – monoclonal antibodies, hormones, peptides, haptens, drugs, etc.
NuGel™ Poly-Amino Technical Data
NuGel™ Poly-Amino is a derivative of NuGel™ polyhydroxy affinity support. This affinity support contains amino groups at the end of hydrophilic spacer arms and is used to couple ligands containing carboxyl, aldehyde, anhydride and epoxy groups.
Spacer Arm: Polymerized hydrophilic carbon chain
Average Particle Size: 50um
Substitution Level: 100-200 uEq/gm of Amino groups
Couples ligands containing free carboxyl groups in the presence of a carbodiimide.
Couples ligands containing free aldehyde, anhydride and epoxy groups.
Couples non-polar ligands in organic solvents.
pH stable from 2 to 9.
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About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com
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NuGel Related References
Chaumet, Alexandre, Sandrine Castella, Laïla Gasmi, Aurélie Fradin, Gilles Clodic, Gérard Bolbach, Robert Poulhe, Philippe Denoulet, and Jean-Christophe Larcher. "Proteomic analysis of Interleukin enhancer binding factor 3 (Ilf3) and Nuclear Factor 90 (NF90) interactome." Biochimie (2013).