Biotech Support Group Release: Delipidation of Bile Samples Using Cleanascite for Comparative Proteomic Analysis
7/15/2013 9:27:18 AM
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PRLog (Press Release) - Jul. 14, 2013 - MONMOUTH JUNCTION, N.J. -- MONMOUTH JUNCTION, N.J. - Cancer biomarkers allow differentiating malignant from nonmalignant biliary stenoses from bile samples via comparative proteomic analysis of bile. Authors Farina et al published an article in the journal Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics titled, 'Bile carcinoembryonic cell adhesion molecule 6 (CEAM6) as a biomarker of malignant biliary stenoses' which cites Cleanascite™ from Biotech Support Group to delipidate bile samples. Bile samples were centrifuged and the supernatant was delipided with Cleanascite™ followed by ultrafiltration. Cleanascite™’s protocol is compatible with samples in which the actual lipid concentration in biological samples varies and different sample volumes are easily scaled. The reagent is a solid-phase, non-ionic adsorbent supplied as a suspension in saline, ready for use. Simply add, centrifuge and/or filter. The clarified supernatant is ready for subsequent downstream processing or analysis. Comparative proteomic biomarker discovery experiments from bile samples of malignant or benign biliary stenosis identified 66 proteins and 7 proteins were elevated in malignant/nonmalignant disease. A cell surface protein, carcinoembryonic cell adhesion molecule 6 (CEAM6), which is associated with cancer was identified via immunoblot. ELISA confirmed CEAM6 as a clinically relevant cancer biomarker of biliary cancers.
For detailed protocols of the published article click
Characteristics of Cleanascite™ Lipid Removal Reagent and Clarification
Helps purify antibodies, recombinant proteins, nucleic acids, proteoglycans
Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
Clarifies saliva and fecal components
Very low protein binding
Does not bind to DNA, RNA, enzymes and proteins
Leaves glycoproteins, antibodies, nucleic acids, hemoglobin, proteoglycans, nucleic acids, serum components(such as hormones, nutrients, globulins, clotting factors, transport proteins) alone
Extends the life of membrane and chromatographic columns.
Enrichment of delipidated tissue samples
Ideal for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monoclonal antibodies.
Selectively removes lipids, cell debris, lipoproteins, floating fats, impurities from cohn paste, transgenic milk, egg yolk and biological samples for pretreatment of samples prior to purification.
For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification
About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com
Biotech Support Group
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Monmouth Junction, NJ 08852, USA
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