Dec 01, 2012 - MONMOUTH JUNCTION, NJ.– Authors Thierry Kamtchoua et al published an article in the journal Vaccine titled, ‘Safety and immunogenicity of the pneumococcal pneumolysin derivative PlyD1 in a single-antigen protein vaccine candidate in adults’ describing the immunogenicity of pneumococcal single antigen protein vaccine in a phase 1, randomized, placebo controlled dose escalating study. Authors cite Cleanascite™ from Biotech Support Group for removal of cholesterol from serum. A toxin neutralizing assay with antibodies in sera was developed to neutralize cytotoxicity caused by Ply in Vero cells. An incubated challenge dose of pneumolysin toxin containing serum diluted with or without Cleanascite™ was developed. The neutralizing titer inhibited the toxin’s effect on Vero cells. According to the paper, “Briefly, the toxin-neutralizing antibody titer was determined by incubating a challenge dose of pneumolysin toxin with serial 2-fold dilutions of serum treated with or without Cleanascite™ HC (Biotech Support Group) to remove cholesterol, an inhibitor of Ply”.
Characteristics Of Cleanascite™
Does not bind to DNA, RNA, enzymes and proteins
Leaves glycoproteins, antibodies, nucleic acids, hemoglobin, proteoglycans, nucleic acids, serum components(such as hormones, nutrients, globulins, clotting factors, transport proteins) alone
Extends the life of membrane and chromatographic columns.
Ideal for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monclonal antibodies.
Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
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Cleanascite™ Lipid Removal Reagent and Clarification
About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com
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