Bio-Rad Laboratories, Inc.'s New Immun-Blot(R) LF PVDF Membranes Improve Fluorescent Blot Detection
Two primary types of protein blotting membranes are used in western blotting applications: nitrocellulose and PVDF (polyvinylidene fluoride). While both types are suitable for colorimetric and chemiluminescent detection, their high autofluorescence and lower signal-to-background ratios are not suitable for fluorescent detection.
Immun-Blot LF PVDF membranes are a low-fluorescence alternative specifically designed for fluorescent immunoblotting and detection in the visible light (red/green/blue) spectrum. Immun-Blot LF PVDF membranes produce highly sensitive and quantitative results thanks to their superior signal-to-background ratios, which are three times higher than those of nitrocellulose and conventional PVDF.
Key Features
Low autofluorescence and high signal-to-background ratio – produce a stronger signal, more accurate protein quantitation, and higher quality images
Superior protein retention – offers binding capacity of 300 µg/cm2 and improves protein retention, especially of low-abundance proteins
Precut membrane and filter paper sets – save time and eliminate excessive membrane handling and potential contamination
Immun-Blot LF PVDF membranes are compatible with any imager possessing visible RGB excitation channels, including Bio-Rad’s new ChemiDoc™ MP imager, which allows researchers to visualize proteins at every stage of a blotting experiment when paired with Bio-Rad’s proprietary Stain-Free technology. The membranes are also compatible with a wide range of fluorescent labels, such as DyLights, Q-Dots, and Alexa fluorophores, as well as colorimetric and chemiluminescent detection methods.
For product details visit http://bit.ly/immunblotlfpvdf .
About Bio-Rad
Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb), has remained at the center of scientific discovery for more than 50 years, manufacturing and distributing a broad range of products for the life science research and clinical diagnostic markets. The company is renowned worldwide among hospitals, universities, major research institutions, as well as biotechnology and pharmaceutical companies for its commitment to quality and customer service. Founded in 1952, Bio-Rad is headquartered in Hercules, California, and serves more than 100,000 research and industry customers worldwide through its global network of operations. The company employs over 6,800 people globally and had revenues exceeding $1.9 billion in 2010. For more information, visit www.bio-rad.com.
For more information contact:
Jeff Xu
Bio-Rad Laboratories, Inc.
510-741-6398
jeff_xu@bio-rad.com
Lawreen Asuncion
Bio-Rad Laboratories, Inc.
510-741-4789
lawreen_asuncion@bio-rad.com
Ken Li
Chempetitive Group
312-997-2436 x 109
kli@chempetitive.com