AnaSpec, Inc. Introduces Industry’s First Fluorimetric Assay Kit for Aggrecanase-1 Activity Detection
Aggrecanases are members of the ADAMTS (A Disintegrin And Metalloprotease with Thrombospondin motif) family of proteases. ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) activities result in the degradation of cartilage aggrecan in arthritic diseases.1-3 ADAMTS-4 (aggrecanase-1) is a major aggrecanase in human osteoarthritic cartilage.4 Other members of the ADAMTS family (ADAMTS-1, -8, -9, -15 and –16) have also been implicated in embryonic development and angiogenesis.5
The kit provides enough reagents to perform 100 assays in a 96-well format. The kit contains: • Aggrecanase-1 FRET substrate • 5-FAM reference standard for calibration • Assay buffer • Aggrecanase inhibitor • Stop Solution.
References :
1. Gur, PK. et al. J. Clin. Invest. 1059, 1335 (2000).
2. Tortorella, MD. Science 284, 1663 (1999).
3. Bonderson, J. Clin. Exp. Rheumatol. 26, 139 (2008).
4. Naito, S, et al. Pathol. Int. 57, 703 (2007).
5. Tang, BL. Int. J. Biochem. Cell Biol. 33, 33 (2001).
About AnaSpec
AnaSpec is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and combinatorial chemistry.
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