Analysis of RNA Polymerase II Mutants using Roche's xCELLigence System

PENZBERG, Germany--(BUSINESS WIRE)--The C-terminal domain (CTD) of RNA Polymerase II consists of multiple repeats of an evolutionary conserved motif of seven amino acids, forming a repetitive structure of 52 repeats in humans and 26 repeats in yeast. Twenty years after the first description of the RNA polymerase II C-terminal domain, its function is still unclear. In a recent research study, M. Heidemann used Roche´s (Pink Sheets: RHHBY) (SWX:RO) (SWX:ROG) xCELLigence RTCA SP Instrument for in vitro testing of cells to test entirely synthetic CTDs. According to his findings, the new xCELLigence System technology allows for the first time the monitoring of cell proliferation and cell death throughout the entire experiment, constantly recording cellular effects during cell plating, transfection, and compound treatment. This non-invasive and continuous monitoring of cells provides a far more detailed picture into the whole experimental process, revealing both specific and off-target effects that may have been missed using conventional endpoint assays.