BioSpace.com

Biotech and Pharmaceutical
News & Jobs
Search the Site
 
   
Biotechnology and Pharmaceutical Channel Medical Device and Diagnostics Channel Clinical Research Channel BioSpace Collaborative    Job Seekers:  Register | Login          Employers:  Register | Login  

NEWSLETTERS
Free Newsletters
Archive
My Subscriptions

NEWS
News by Subject
News by Disease
News by Date
PLoS
Search News
Post Your News
JoVE

CAREER NETWORK
Job Seeker Login
Most Recent Jobs
Browse Biotech Jobs
Search Jobs
Post Resume
Career Fairs
Career Resources
For Employers

HOTBEDS
Regional News
US & Canada
  Biotech Bay
  Biotech Beach
  Genetown
  Pharm Country
  BioCapital
  BioMidwest
  Bio NC
  BioForest
  Southern Pharm
  BioCanada East
  US Device
Europe
Asia

DIVERSITY

INVESTOR
Market Summary
News
IPOs

PROFILES
Company Profiles

START UPS
Companies
Events

INTELLIGENCE
Research Store

INDUSTRY EVENTS
Biotech Events
Post an Event
RESOURCES
Real Estate
Business Opportunities

 News | News By Subject | News by Disease News By Date | Search News
eNewsletter Signup
Miles
Km80.5

   

Algorithme Pharma Uses HRMS to Successfully Analyze Intact TRAIL


6/28/2013 9:01:36 AM

Get the latest biotech news where you want it. Sign up for the free GenePool newsletter today!

June 2013 -- TRAIL (tumor necrosis factor-related apoptosis-inducing ligand also known as APO2L - TNF superfamily) is the first recombinant human protein that selectively induces apoptosis in cancer cells while sparing normal cells. In addition to its potential anti-cancer properties, TRAIL also demonstrates numerous other potential therapeutic uses in cardiovascular diseases, diabetes and atherosclerosis. These developments make TRAIL a promising target in the field of biotherapeutics.

In developing a method, multiple challenges have to be overcome including chromatographic separation, low sensitivity and high signal variability. High Resolution Mass Spectrometry (HRMS) offers an alternative platform in quantifying TRAIL to support pharmacokinetic investigations.

To obtain successful chromatographic separation, Algorithme Pharma used a polymer based column which improved the peak shape and injection reproducibility. To increase the column recovery, the temperature was raised which resulted in a quadrupled recovery rate. The data processing method was then optimized to avoid interferences from endogenous compounds. To overcome the low sensitivity and high signal variability, the 6 most abundant multiple charge states were summed with an XIC window of 10mDa around the most abundant isotopomer.

Algorithme Pharma has successfully overcome the multiple challenges involved in using HRMS to quantify intact TRAIL and has confirmed its applicability as an optimal platform for intact protein quantification without the need for tryptic digestion.

For more information regarding our bioanalytical services, please contact us at contact@algopharm.com.

Help employers find you! Check out all the jobs and post your resume.


Read at BioSpace.com

   

ADD TO DEL.ICIO.US    ADD TO DIGG    ADD TO FURL    ADD TO STUMBLEUPON    ADD TO TECHNORATI FAVORITES