BioSpace.com

Biotech and Pharmaceutical
News & Jobs
Search the Site
 
   
Biotechnology and Pharmaceutical Channel Medical Device and Diagnostics Channel Clinical Research Channel BioSpace Collaborative    Job Seekers:  Register | Login          Employers:  Register | Login  

NEWSLETTERS
Free Newsletters
Archive
My Subscriptions

NEWS
News by Subject
News by Disease
News by Date
PLoS
Search News
Post Your News
JoVE

CAREER NETWORK
Job Seeker Login
Most Recent Jobs
Browse Biotech Jobs
Search Jobs
Post Resume
Career Fairs
Career Resources
For Employers

HOTBEDS
Regional News
US & Canada
  Biotech Bay
  Biotech Beach
  Genetown
  Pharm Country
  BioCapital
  BioMidwest
  Bio NC
  BioForest
  Southern Pharm
  BioCanada East
  US Device
Europe
Asia

DIVERSITY

INVESTOR
Market Summary
News
IPOs

PROFILES
Company Profiles

START UPS
Companies
Events

INTELLIGENCE
Research Store

INDUSTRY EVENTS
Biotech Events
Post an Event
RESOURCES
Real Estate
Business Opportunities

 News | News By Subject | News by Disease News By Date | Search News
eNewsletter Signup
Miles
Km80.5

   

Agilent Automation Solutions Release: Automation of a Magnetic Bead Workflow for Protein Biomarker Quantification


5/3/2011 9:53:48 AM

03 May 2011 -- Agilent Automation Solutions has released a new application note that demonstrates how its Bravo liquid handling system provides an efficient platform for automation of a SISCAPA Magnetic Bead Workflow for protein biomarker quantification by Mass Spectrometry.

Mass Spectrometry (MS) has become the benchmark technology for analysis of peptides based upon its ability to deliver precise quantification, near absolute structural specificity and multiplexing of measurements in a single analytical run. These advantages can be exploited in the analysis of peptides which occur in a single sequence to reveal the concentration of the parent proteins in digests of complex samples such as patient plasma or serum.

The authors of this new application note describe an automated protocol implementing SISCAPA immunoaffinity enrichment of biomarker peptides prior to quantification by MS. In the described protocol, magnetic beads coated with anti-peptide antibodies were used to bind specific target peptides from tryptic digests of plasma and serum samples, after which the beads were washed to remove unbound peptides, and the bound, purified peptides were eluted in small volumes for injection and analysis in an LC/MS/MS system. Stable isotope labeled versions of the same peptides was used as internal standards to enable accurate quantification. The Agilent Bravo automated protocol for this application enabled SISCAPA processing of 96 samples in less than 30 minutes, less than half the time required by traditional manual methods.

For a copy of this application note visit www.chem.agilent.com/Library/applications/5990-7360en_lo%20CMS.pdf or contact your local Agilent sales office.

From stand-alone units to fully integrated systems, Agilent Automation Solutions teams key sample preparation and creative walk-away automation approaches with personalized customer service to provide complete solutions for your laboratory. Combining innovative engineering with high standards of quality, Agilent designs and manufactures high-performance equipment for processes that are revolutionizing pharmaceutical, biotech, and genomic research.

Europe

Agilent Automation Solutions

New Cambridge House

Bassingbourn Road

Litlington, Royston

Hertfordshire SG8 0SS UK

tel. +44-1763-850230

email europeansales.automation@agilent.com

www.agilent.com/lifesciences/automation

Worldwide Headquarters

Agilent Automation Solutions

5301 Stevens Creek Blvd.

Santa Clara, CA 95051

USA

tel. +1 408 553 2424

email todd_christian@agilent.com

www.agilent.com/lifesciences/automation

Media Contact: (Primetek Solutions)

tel. +44-208-546-0869

email info@primetek-solutions.com



Read at BioSpace.com

 
 

ADD TO DEL.ICIO.US    ADD TO DIGG    ADD TO FURL    ADD TO STUMBLEUPON    ADD TO TECHNORATI FAVORITES